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Expression of Antibody in Mammalian Cells

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Antibody Expression and Production

Part of the book series: Cell Engineering ((CEEN,volume 7))

Abstract

Despite having a reputation to be costly, mammalian cell culture processes are used to produce the majority of currently marketed recombinant biopharmaceuticals, many of which are antibodies. Historically, mammalian cells were mainly chosen for whole IgG manufacturing because of product quality requirements like e.g. glycosylation, folding and assembly of the individual chains, which make microbial expression difficult. However, during the past 2 decades, significant progress has been made in both: Speed of mammalian cell line generation and yield of the manufacturing processes, which makes mammalian systems also from a commercial point of view more and more competitive to microbial expression. The following chapter provides an overview of state of the art mammalian cell line technologies for antibody manufacturing starting from vector and selection systems over host cell lines to screening methods.

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Acknowledgements

I would like to thank Hans-Peter Knopf and Burkhard Wilms for their help and for proof-reading of the manuscript.

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  1. Novartis Pharma AG, CH-4002, Basel, Switzerland

    Thomas Jostock

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  1. Thomas Jostock
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Correspondence to Thomas Jostock .

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  1. Dept. Chemical & Biochemical, Engineering, University College, Dublin, Belfield, Dublin 4, Ireland

    Mohamed Al-Rubeai

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Jostock, T. (2011). Expression of Antibody in Mammalian Cells. In: Al-Rubeai, M. (eds) Antibody Expression and Production. Cell Engineering, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1257-7_1

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