Abstract
This paper describes key examples of our work on biomaterial integration for functionalization and characterization in microdevices. We use the polysaccharide chitosan for immobilization of DNA and proteins in micro-fabricated sensors to make them responsive to a particular sample. In some cases, the use of chitosan was shown to improve the detection signal by a factor of 100 compared to devices without chitosan. We have developed a method for depositing Tobacco mosaic virus on micro scale electrodes in order to increase the effective surface area in a battery. Consequently, the capacity was increased by a factor of 6 compared to devices with planar electrodes. We have demonstrated the culturing of bacteria in a microfluidic test platform. The growth of bacteria over time is measured optically to provide information about the bacterial response to different stimuli. In a related demonstration, bacterial enzymes are assembled in a fluidic channel and their products under varying conditions are detected with a microcantilever sensor. The use of microfabricated devices for these experiments enables high-density, high-throughput measurements of the biomaterials to be performed.
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Acknowledgements
The authors would like to thank the R.W. Deutsch Foundation and the National Science Foundation Emerging Frontiers in Research and Innovation (NSF-EFRI) for funding this work. This work has been supported in part by the Laboratory for Physical Sciences. We also appreciate the support of the Maryland NanoCenter and its FabLab.
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Ghodssi, R. et al. (2010). Integration of Diverse Biological Materials in Micro/Nano Devices. In: Gusev, E., Garfunkel, E., Dideikin, A. (eds) Advanced Materials and Technologies for Micro/Nano-Devices, Sensors and Actuators. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3807-4_22
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