Skip to main content

Chitin Metabolism In Fungi

  • Chapter
Progress in Mycology
  • 2539 Accesses

Abstract

The fungal kingdom is very diverse, with species growing as unicellular yeasts to branching hyphae. Developing an outer protective layer, namely the cell wall, is critical for growth and survival of the fungal cell in the diverse environments where fungi live. The shape and integrity of the fungus is dependent upon the mechanical strength of the cell wall, which performs a wide range of essential roles during the interaction of the fungus with its environment. The wall composition frequently varies markedly between different species of fungi (Adams, 2004). The fungal cell wall is a complex structure composed of chitin, glucans and other polymers, and there is evidence of extensive cross- linking between these components. The complexity of the cell wall means that its biogenesis demands a significant number of cellular activities that have to act in concert with the essential functions controlling cell growth and morphogenesis, since the wall determines cell shape. Furthermore, the wall is a highly dynamic structure subject to constant change, for example, during cell expansion and division in yeasts, and during spore germination, hyphal branching and septum formation in filamentous fungi. Cell wall polymer branching, cross-linking, and the maintenance of wall plasticity during morphogenesis, may depend upon the activities of a range of hydrolytic enzymes found intimately associated with the fungal cell wall (Dahiya, 2007).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Adams, D.J. (2004). Fungal cell wall chitinases and glucanases. Microbiol 150, 2029–2035.

    Article  CAS  Google Scholar 

  • Altamirano, M.M., J.M. Blackburn, C. Agyayo and AR Fersht (2000). Directed evolution of new catalytic activity using the αβ-barrel scaffold, Nature 403: 617–622.

    Article  CAS  PubMed  Google Scholar 

  • Arai, N., K. Shiomi, Y. Yamaguchi, R. Masuma, Y. Iwai, A. Turberg, H. Kolbl and S. Omura (2000). Argadin, a new chitinase inhibitor, produced by Clonostachys sp. FO-7314, Chem Pharm Bull 48:1442–1446.

    CAS  PubMed  Google Scholar 

  • Boer, H., N. Munck, J. Natunen, G. Wohlfahrt, H. Soderlund, O.Renkonen and A. Koivula (2004). Differential recognition of animal type beta 4-galactosylated and alpha3-fucosylated chito-oligosaccharides by two family 18 chitinases from Trichoderma harzianum, Glycobiol 14:1303–1313.

    Article  CAS  Google Scholar 

  • Boga, M.R., C.A Rota, H.J. Pinto, M. Ocampos, C.T. Correa, M.H. Vainstein. and A. Schrank (1998). A chitinase encoding gene (chitl gene) from the entomopathogen Metarhizium anisopliae: isolation and characterization of genomic and full-length cDNA, Curr Microbionl 37: 221–225.

    Article  Google Scholar 

  • Bonfim, S.M., A.H. Cruz, R.S. Jesuino, C.J. Ulhoa, E.E. Molinari-Madlum, C.M. Soares and M. Pereira (2006). Chitinase from Paracoccidioides brasiliensis: molecular cloning, structural, phylogenetic, expression and activity analysis, FEMS Immunol Med Microbiol 46: 269–283.

    Article  CAS  PubMed  Google Scholar 

  • Brameld, K.A., W.D. Shrader, B. Imperiali and W.A. Goddard (1998). Substrate assistance in the mechanism of family 18 chitinases: theoretical studies of potential intermediates and inhibitors, J Mol Biol 280: 913–923.

    Article  CAS  PubMed  Google Scholar 

  • Brunner, K., M. Montero, R.L. Mach, C.K. Peterbauer and C.P. Kubicek (2003). Expression of the ech42 (endochitinase) gene of Trichoderma atroviride under carbon starvation is antagonized via a BrlA-like cis-acting element, FEMS Microbiol Lett 218: 259–264.

    Article  CAS  PubMed  Google Scholar 

  • Cohen, E. (2001). Chitin synthesis and inhibition: a revisit, Pest Manag Sci 57: 946–950.

    Article  CAS  PubMed  Google Scholar 

  • da Silva, M.V., L. Santi, C.C. Staats, A.M. da Costa, E.M. Colodel, D. Driemeier, M.H. Vainstein and A. Schrank (2005). Cuticle-induced endo/exoacting chitinase CHIT30 from Metarhizium anisopliae is encoded by an ortholog of the chi3 gene, Res Microbiol 156:382–392.

    Article  PubMed  Google Scholar 

  • Dahiya, N. (2007). Fungal chitinases An overview on regulation and cloning. In: “Mycotechnology: Present status and future prospects” Rai M. (Editor), I.K. International Publishing House, India, pp 106–120.

    Google Scholar 

  • Dahiya, N., R. Tiwari and G.S. Hoondal (2005). Chitinase from Enterobacter sp. NRG4: Its purification, characterization and reaction pattern, Elec J Biotechnol 15: 134–145.

    Article  Google Scholar 

  • Djebaili, M. and J. B. Behr (2005). Potent inhibition of chitin synthase by an azasugar investigation of synergistic effect with UDP, J Enzyme Inhib Med Chem 20:123–127.

    Article  CAS  PubMed  Google Scholar 

  • El-Katatny, M.H., M. Gudelj, K.H. Robra, M.A. Elnaghy and G.M. Gubitz (2001). Characterizatioin of a chitinase and endo β-1,3 glucanase from Trichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii, Appl Microbiol Biotechnol 56:137–143.

    Article  CAS  PubMed  Google Scholar 

  • Escort, G.M., V.M. Hearn and D.J. Adams (1998). Inducible chitinolytic system of Aspergillus fumigatus, Microbiol 144:1575–1581.

    Article  Google Scholar 

  • Fang, W., B. Leng, Y. Xiao, K. Jin, J. Ma, Y. Fan, J. Feng, X. Yang, Y. Zhang and Y. Pei (2005). Cloning of Beauveria bassiana chitinase gene Bbchitl and its application to improve fungal strain virulence, Appl Environ Microbiol 71: 363–370.

    Article  CAS  PubMed  Google Scholar 

  • Filho, B.P., F.J. Lemos, N.F. Secundino, V. Pascoa, S.T. Pereira and P.F. Pimenta (2002). Presence of chitinase and beta-N-acetylglucosaminidase in the Aedes aegypti: a chitinolytic system involving peritrophic matrix formation and degradation, Insect Biochem Mol Biol 32: 1723–1729.

    Article  CAS  PubMed  Google Scholar 

  • Fournet, F., C. Sannier, M. Moriniere, P. Porcheron and N. Monteny (1995). Effects of two insect growth regulators on ecdysteroid production in Aedes aegypti (Diptera: Culicidae), J Med Entomol 32:588–593.

    CAS  PubMed  Google Scholar 

  • Gardner, K.H. and J. Blackwell (1975). Refinement of the structure of β-chitin, Biopol 14:1581–1595.

    Article  CAS  Google Scholar 

  • Gkargkas, K., D. Mamma, G. Nedey, E. Topakas, P. Christakopoulos, D. Kekos and B.J. Macris (2003). Studies on a N-acetyl-β-D-glucosaminidase produced by Fusarium oxysporum F3 grown in solid-state fermentation, Proc Biochem 39:1599–1605.

    Article  Google Scholar 

  • Glaser, L. and D. H. Brown (1957). The synthesis of chitin in cell-free extracts of Neurospora crassa, J Biol Chem 228: 729–742.

    CAS  PubMed  Google Scholar 

  • Henrissat, B. and G. Davies (1997). Structural and sequence-based classification of glycoside hydrolases, Curr Opin Struct Biol 7: 637–644.

    Article  CAS  PubMed  Google Scholar 

  • Herrera-Estrella, A. and I. Chet (1999). Chitinases in biological control. EXS 87, 171–184.

    CAS  PubMed  Google Scholar 

  • Herzberg, O. and Moult, J. (1991). Analysis of the steric strain in the polypeptide backbone of protein molecules, Proteins Struct Funct Genet 11: 223–229.

    Article  CAS  PubMed  Google Scholar 

  • Houston, D.R., I. Eggleston, B. Synstad, V.G. Eijsink and D.M. van Aalten (2002). The cyclic dipeptide CI-4 [cyclo-(1-Arg-d-Pro)] inhibits family 18 chitinases by structural mimicry of a reaction intermediate, Biochem J 368: 23–27.

    Article  CAS  PubMed  Google Scholar 

  • Hwang, E.I., B.T. Ahn, H.B. Lee, Y.K. Kim, K.S. Lee, S.H. Bok, Y.T. Kim and S.U. Kim (2001). Inhibitory activity for chitin synthase II from Saccharomyces cerevisiae by tannins and related compounds, Planta Med 67: 501–504.

    Article  CAS  PubMed  Google Scholar 

  • Hwang, E.I., B.M. Kwon, S.H. Lee, N.R. Kim, T.H. Kang, Y.T. Kim, B.K. Park and S.U. Kim (2002). Obovatols, new chitin synthase 2 inhibitors of Saccharomyces cerevisiae from Magnolia obovata, J Antimicrob Chemother 49: 95–101.

    Article  CAS  PubMed  Google Scholar 

  • Hwang, E.I., B.S. Yun, Y.K. Kim, B.M Kwon, H.G. Kim, H.B. Lee, W.J. Jeong and S.U. Kim (2000). Phellinsin A, a novel chitin synthases inhibitor produced by Phellinus sp. PL3, J Antibiot (Tokyo) 53: 903–911.

    CAS  Google Scholar 

  • Jaques, A. K., T. Fukamizo, D. Hall, R.C. Barton, G.M. Escott, T. Parkinson, C.A. Hitchcock and D.J. Adams (2003). Disruption of the gene encoding the ChiB1 chitinase of Aspergillus fumigatus and characterization of a recombinant gene product, Microbiol 149: 2931–2939.

    Article  CAS  Google Scholar 

  • Jeong, T.S., E.I. Hwang, H.B. Lee, E.S. Lee, Y.K. Kim, B.S. Min, K.H. Bae, S.H. Bok and S.U. Kim (1999). Chitin synthase II inhibitory activity of ursolic acid, isolated from Crataegus pinnatifida, Planta Med 65:261–263.

    Article  CAS  PubMed  Google Scholar 

  • Kawachi, I., T. Fujieda, M. Ujita, Y. Ishii, K. Yamagishi, H. Sato, T. Funaguma and A. Hara (2001). Purification and properties of extracellular chitinases from the parasitic fungus Isariajaponica, J Biosci Bioeng 92: 544–549.

    Article  CAS  PubMed  Google Scholar 

  • Kim, M.K., H.S. Park, C.H. Kim, H.M. Park and W. Choi (2002b). Inhibitory effect of nikkomycin Z on chitin synthases in Candida albicans, Yeast 19: 341–349.

    Article  CAS  PubMed  Google Scholar 

  • Kim, D.J., J.M. Baek, P. Uribe, C.M. Kenerley and D.R. Cook (2002a). Cloning and characterization of multiple glycosyl hydrolase genes from Trichoderma virens, Curr Genet 40: 374–384.

    Article  CAS  PubMed  Google Scholar 

  • Kim, S.U., E.I. Hwang, J.Y. Nam, K.H. Son, S.H. Bok, H.E. Kim and B.M. Kwon (1999). Inhibition of chitin synthase II by catechins from stem bark of Taxus cuspidate, Planta Med 65: 97–98.

    Article  CAS  PubMed  Google Scholar 

  • Kramer, K.J. and D. Koga (1986). Insect chitin: physical state, synthesis, degradation and metabolic regulation, Insect Biochem 16: 851–877.

    Article  CAS  Google Scholar 

  • Leloir, L.F. and C.E. Cardini (1953). The biosynthesis of glucosamine, Biochim Biophys Acta 12:15–22.

    Article  CAS  PubMed  Google Scholar 

  • Lesage, G., J. Shapiro, C. A. Specht, A.M. Sdicu, P. Menard, S. Hussein, A.H. Tong, C. Boone and H. Bussey (2005). An interactional network of genes involved in chitin synthesis in Saccharomyces cerevisiae, BMC Genet 6:8.

    Article  PubMed  Google Scholar 

  • Machida, S. and M. Saito (1993). Purification and characterization of membrane-bound chitin synthase, J Biol Chem 268:1702–1707.

    CAS  PubMed  Google Scholar 

  • Maia, J.C. (1994). Hexosamine and cell wall biogenesis in the aquatic fungus Blastocladiella emersonii, FASEB J 8: 848–853.

    CAS  PubMed  Google Scholar 

  • Mayer, R.T., A.C. Chen and J.R. DeLoach (1980). Characterization of a chitin synthase from the stable fly, Stomoxys calcitrans (L.), Insect Biochem 10: 549–556.

    Article  CAS  Google Scholar 

  • Mercedes Dana, M., M.C. Limón, R. Mejias, L.M. Robert, B. Tahía, A.P.T. José and C.P. Kubicek (2001). Regulation of chitinase 33 (chit33) gene expression in Trichoderma harzianum, Curr Gen 38: 335–342.

    Article  Google Scholar 

  • Omura, S., N. Arai, Y. Yamaguchi, R. Masuma, Y. Iwai, M. Namikoshi, A. Turberg, H. Kolbl and K. Shiomi (2000). Argifin, a new chitinase inhibitor, produced by Gliocladium sp. FTD-0668. I. Taxonomy, fermentation, and biological activities, J Antibio 53: 603–608.

    CAS  Google Scholar 

  • Peter, M.G. (2004). Chitin and chitosan in Fungi. Polysaccharides II: Polysaccharides from Eukaryotes. In: “Biopolymers (De Baets, S., Vandamme, E. and Steinbüchel, A.). Vol. 6, pp. 123–147. Interscience WILEY-VCH.

    Google Scholar 

  • Pinto, Ades., Barreto, C.C., A. Schrank, C.J. Ulhao and M.H. Vainstein (1997). Purification and characterization of an extracellular chitinase from the entomopathogen Metarhizium anisopliae, Can J Microbiol 43: 322–327.

    Article  CAS  Google Scholar 

  • Pishko, E.J., T.N. Kirkland and G.T. Cole (1995). Isolation and characterization of two chitinase-encoding genes (ctsl, cts2) from the fungus Coccidioides immitis, Gene 167: 173–177.

    Article  CAS  PubMed  Google Scholar 

  • Ramakrishanan, C. and N. Prasad (1972). Rigid body refinement and conformation of a-chitin, Biochim Biophys Acta 261:123–135.

    Google Scholar 

  • Rao, F.V., O.A. Andersen, K.A. Vora, J.A. Demartino and D.M. van Aalten (2005). Methylxanthine drugs are chitinase inhibitors: investigation of inhibition and binding modes, Chem Biol 12, 973–980.

    Article  CAS  PubMed  Google Scholar 

  • Roncero, C. (2002). The genetic complexity of chitin synthesis in fungi, Curr Genet 41: 367–378.

    Article  CAS  PubMed  Google Scholar 

  • Sakuda, S., A. Isogai, S. Matsumoto and A. Suzuki (1987). Search for microbial insect growth regulators. II. Allosamidin, a novel insect chitinase inhibitor, J Antibiot 40: 296–300.

    CAS  PubMed  Google Scholar 

  • Sakurda, M., D.P. Morgavi, K. Komatani, Y. Tomita and R. Onodera (1996). Purification and characterization of a cvtosolic chitinase from Piromyces communite OTSI, FEMS Microhiol Lett 137: 75–78.

    Article  Google Scholar 

  • Seidl, V., B. Huemer, B. Seiboth. M.C.P. Kubicek (2005). A complete survey of Trichoderma chitinases reveals three distinct subgroups of family 18 chitinases, FEBS J 272:5923–5939.

    Article  CAS  PubMed  Google Scholar 

  • Souza, R.F., R.C. Gomes, R.R. Coelho, C.S. Alviano and R.M. Soares (2003). Purification and characterization of an endochitinase produced by Colletotrichum gloeosporioides, FEMS Microbiol Lett 222: 45–50.

    Article  CAS  PubMed  Google Scholar 

  • Takaya, N., D. Yamazaki, H. Horiuchi, A. Ohta and M. Takagi (1998). Cloning and characterization of a chitinase-encoding gene (chiA) from Aspergillus nidulans, disruption of which decreases germination frequency and hyphal growth, Biosci Biotechnol Biochem 62: 60–65.

    Article  CAS  PubMed  Google Scholar 

  • Takaya, N., D. Yamazaki, H. Horiuchi, A. Ohta and M. Takagi (1998). Intracellular chitinase gene from Rhizopus oligosporus: molecular cloning and characterization, Microbiol 144: 2647–2654.

    Article  CAS  Google Scholar 

  • Tews, I., A.C. Scheltinga, V. Terwisscha, A. Perrakis, K.S. Wilson and B.W. Dijkstra (1997). Substrate assisted catalysis unifies two families of chitinolytic enzymes, J Amer Chem Soc 119: 7954–7959.

    Article  CAS  Google Scholar 

  • Uchida, Y., O. Shimmi, M. Sudoh, M. Arisawa, H. Yamada-Okabe (1996). Characterization of chitin synthase 2 of Saccharomyces cerevisiae. II: Both full size and processed enzymes are active for chitin synthesis, J Biochem (Tokyo) 119: 659–666.

    CAS  Google Scholar 

  • Ulhoa, C.J. and J.F. Peberdy (1991). Regulation of chitinase synthesis in Trichoderma harzianum, J Gen Microbiol 137: 2193–2169.

    Google Scholar 

  • Vaaje-Kolstad, G., A. Vasella, M.G. Peter, C. Netter, D.R. Houston, B. Westereng, B. Synstad, V.G. Eijsink and D.M. van Aalten (2004). Interactions of a family 18 chitinase with the designed inhibitor HM508 and its degradation product, chitobiono-delta-lactone, J Biol Chem 279: 3612–3619.

    Article  CAS  PubMed  Google Scholar 

  • van Aalten, D.M., D. Komander, B. Synstad, S. Gaseidnes, M.G. Peter and V.G. Eijsink (2001). Structural insights into the catalytic mechanism of a family 18 exochitinase, Proc Natl Acad Sci USA 98: 8979–8984.

    Article  PubMed  Google Scholar 

  • van Eck, W.H. (1979). Mode of action of two benzoylphenyl ureas as inhibitors of chitin synthesis in insects, Insect Biochem 9: 295–300.

    Article  Google Scholar 

  • Vasseur, V., F. Arigoni, H. Andersen, G. Defago, G. Bompeix and J.M. Seng (1990). Isolation and characterization of Aphanocladium album chitinase overproducing mutants J Gen Microbiol 136: 2561–2567.

    CAS  Google Scholar 

  • Viterbo, A., M. Montero, O. Ramot, D. Friesem, E. Monte, A. Llobell and I. Chet (2002). Expression regulation of the endochitinase chit36 from Trichoderma asperellum (T. harzianum T-203), Curr Gene 42: 114–122.

    Article  CAS  Google Scholar 

  • Zhang, D. and M.J. Miller (1999). Polyoxins and nikkomycins: progress in synthetic and biological studies, Curr Pharm Des 5: 73–99.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2010 Scientific Publishers (India)

About this chapter

Cite this chapter

Dahiya, N. (2010). Chitin Metabolism In Fungi. In: Rai, M., Kövics, G. (eds) Progress in Mycology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3713-8_15

Download citation

Publish with us

Policies and ethics