Abstract
In this chapter, we will introduce the methods for the generation of transgenic rabbits. These methods are those used for transgenic mice but with some modifications and refinements. We will review all possible methods for rabbit transgenesis with emphasis on microinjection method because it is the most popular method so far.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Al-Hasani, S., Tolksdorf, A., Diedrich, K., Van Der Ven, H. and Krebs, D. (1986) Successful in-vitro fertilization of frozen-thawed rabbit oocytes. Hum Reprod 1:309–312.
Baguisi, A., Behboodi, E., Melican, D.T., Pollock, J.S., Destrempes, M.M., Cammuso, C., Williams, J.L., Nims, S.D., Porter, C.A., Midura, P., Palacios, M.J., Ayres, S.L., Denniston, R.S., Hayes, M.L., Ziomek, C.A., Meade, H.M., Godke, R.A., Gavin, W.G., Overstrom, E.W. and Echelard, Y. (1999) Production of goats by somatic cell nuclear transfer. Nat Biotechnol 17:456–461.
Bosze, Z., Hiripi, L., Carnwath, J.W. and Niemann, H. (2003) The transgenic rabbit as model for human diseases and as a source of biologically active recombinant proteins. Transgenic Res 12:541–553.
Brackett, B.G., Baranska, W., Sawicki, W. and Koprowski, H. (1971) Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. Proc Natl Acad Sci U S A 68:353–357.
Cheng, H., Dooley, M.P., Hopkins, S.M., Anderson, L.L., Yibchok-Anun, S. and Hsu, W.H. (1999) Development of rabbit embryos during a 96-h period of in vitro culture after supero-vulatory treatment under conditions of elevated ambient temperature. Anim Reprod Sci 56:279–290.
Chesne, P., Adenot, P.G., Viglietta, C., Baratte, M., Boulanger, L. and Renard, J.P. (2002) Cloned rabbits produced by nuclear transfer from adult somatic cells. Nat Biotechnol 20:366–369.
Cibelli, J.B., Stice, S.L., Golueke, P.J., Kane, J.J., Jerry, J., Blackwell, C., Ponce De Leon, F.A. and Robl, J.M. (1998) Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280:1256–1258.
Dull, T., Zufferey, R., Kelly, M., Mandel, R.J., Nguyen, M., Trono, D. and Naldini, L. (1998) A third-generation lentivirus vector with a conditional packaging system. J Virol 72:8463–8471.
Fan, J. and Watanabe, T. (2003) Transgenic rabbits as therapeutic protein bioreactors and human disease models. Pharmacol Ther 99:261–282.
Fan, J., Challah, M. and Watanabe, T. (1999) Transgenic rabbit models for biomedical research: current status, basic methods and future perspectives. Pathol Int 49:583–594.
Gordon, J.W., Scangos, G.A., Plotkin, D.J., Barbosa, J.A. and Ruddle, F.H. (1980) Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci U S A 77:7380–7384.
Hammer, R.E., Pursel, V.G., Rexroad, C.E., Jr., Wall, R.J., Bolt, D.J., Ebert, K.M., Palmiter, R.D. and Brinster, R.L. (1985) Production of transgenic rabbits, sheep and pigs by microinjection. Nature 315:680–683.
Hashimoto, S., Kuramochi, T., Aoyagi, K., Takahashi, R., Ueda, M., Hirao, M., Kamei, M., Kitada, K. and Hirasawa, K. (2004) Refined porcine follicle stimulating hormone promotes the responsiveness of rabbits to multiple-ovulation treatment. Exp Anim 53:395–397.
Hashimoto, S., Kimura, K., Kuramochi, T., Aoyagi, K., Hirako, M., Kawaguchi, M., Iwata, H., Hirao, M., Kitada, K., Hirasawa, K. and Ueda, M. (2007) Responsiveness of rabbits to superovulation treatment by a single injection of follicle-stimulating hormone with aluminum hydroxide gel. Mol Reprod Dev 74:1208–1212.
Hofmann, A., Kessler, B., Ewerling, S., Weppert, M., Vogg, B., Ludwig, H., Stojkovic, M., Boelhauve, M., Brem, G., Wolf, E. and Pfeifer, A. (2003) Efficient transgenesis in farm animals by lentiviral vectors. EMBO Rep 4:1054–1060.
Hofmann, A., Zakhartchenko, V., Weppert, M., Sebald, H., Wenigerkind, H., Brem, G., Wolf, E.and Pfeifer, A. (2004) Generation of transgenic cattle by lentiviral gene transfer into oocytes. Biol Reprod 71:405–409.
Kauffman, R.D., Schmidt, P.M., Rall, W.F. and Hoeg, J.M. (1998) Superovulation of rabbits with FSH alters in vivo development of vitrified morulae. Theriogenology 50:1081–1092.
Keefer, C.L., Bennett, K.A. and Brackett, B.G. (1985) In vitro fertilization in the rabbit after delayed ovum recovery. Biol Reprod 33:388–392.
Kitajima, S., Morimoto, M., Watanabe, T. and Fan, J. (2003) Transgenic Rabbits: Production and Application for Human Disease Models. Recent Res Devel Biophys Biochem 3:179–194.
Lai, L., Kolber-Simonds, D., Park, K.W., Cheong, H.T., Greenstein, J.L., Im, G.S., Samuel, M., Bonk, A., Rieke, A., Day, B.N., Murphy, C.N., Carter, D.B., Hawley, R.J. and Prather, R.S. (2002) Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295:1089–1092.
Lavitrano, M., Camaioni, A., Fazio, V.M., Dolci, S., Farace, M.G. and Spadafora, C. (1989) Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice. Cell 57:717–723.
Li, L., Shen, W., Min, L., Dong, H., Sun, Y. and Pan, Q. (2006) Human lactoferrin transgenic rabbits produced efficiently using dimethylsulfoxide-sperm-mediated gene transfer. Reprod Fertil Dev 18:689–695.
Lois, C., Hong, E.J., Pease, S., Brown, E.J. and Baltimore, D. (2002) Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295:868–872.
Mccreath, K.J., Howcroft, J., Campbell, K.H., Colman, A., Schnieke, A.E. and Kind, A.J. (2000) Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 405:1066–1069.
Mcgrew, M.J., Sherman, A., Ellard, F.M., Lillico, S.G., Gilhooley, H.J., Kingsman, A.J., Mitrophanous, K.A. and Sang, H. (2004) Efficient production of germline transgenic chickens using lentiviral vectors. EMBO Rep 5:728–733.
Moore, N.W., Adams, C.E. and Rowson, L.E. (1968) Developmental potential of single blast-omeres of the rabbit egg. J Reprod Fertil 17:527–531.
Pfeifer, A., Ikawa, M., Dayn, Y. and Verma, I.M. (2002) Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci USA 99:2140–2145.
Shen, W., Li, L., Pan, Q., Min, L., Dong, H. and Deng, J. (2006) Efficient and simple production of transgenic mice and rabbits using the new DMSO-sperm mediated exogenous DNA transfer method. Mol Reprod Dev 73:589–594.
Smith, K. and Spadafora, C. (2005) Sperm-mediated gene transfer: applications and implications. Bioessays 27:551–562.
Van Den Brandt, J., Wang, D., Kwon, S.H., Heinkelein, M. and Reichardt, H.M. (2004) Lentivirally generated eGFP-transgenic rats allow efficient cell tracking in vivo. Genesis 39:94–99.
Wakayama, T., Perry, A.C., Zuccotti, M., Johnson, K.R. and Yanagimachi, R. (1998) Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369–374.
Wilmut, I., Schnieke, A.E., Mcwhir, J., Kind, A.J. and Campbell, K.H. (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385:810–813.
Zheng, Y.L., Jiang, M.X., Zhang, Y.L., Sun, Q.Y. and Chen, D.Y. (2004) Effects of oocyte age, cumulus cells and injection methods on in vitro development of intracytoplasmic sperm injection rabbit embryos. Zygote 12:75–80.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science + Business Media B.V.
About this chapter
Cite this chapter
Kitajima, S., Liu, E., Fan, J. (2009). Rabbit Transgenesis. In: Houdebine, LM., Fan, J. (eds) Rabbit Biotechnology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2227-1_6
Download citation
DOI: https://doi.org/10.1007/978-90-481-2227-1_6
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-2226-4
Online ISBN: 978-90-481-2227-1
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)