Endoglycosidases (Glycoproteins)

  • Kaoru Takegawa
  • Kenji Yamamoto


Endo-β-N-acetylglucosaminidase (EC hydrolyzes the glycosidic bond in the N, N′-diacetylchitobiose moiety of N-linked oligosaccharides of various glycoproteins. This enzyme is unique with regard to the enzymatic action by which one GlcNAc residue remains bound to the protein/peptide.


Glycoside Hydrolase Family Sugar Chain Transglycosylation Activity GlcNAc Residue Transglycosylation Product 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Fujita K, Kobayashi K, Iwamatsu A, Takeuchi M, Natsuka S, Ikura K, Kumagai H, Yamamoto K (2004) Molecular cloning and characterization of Mucor hiemalis endo-β-N-acetylglucosaminidase that can transglycosylate complex type N-linked oligosaccharides. Arch Biochem Biophys 432:41–49PubMedGoogle Scholar
  2. Haneda K, Inazu T, Mizno M, Yamamoto K (2003) Chemoenzymatic synthesis of neoglycoconjugates by transglycosylation with endo-β-N-acetylglucosaminidase from Mucor hiemalis. Methods Enzymol 362:74–85PubMedCrossRefGoogle Scholar
  3. Takegawa K, Fan JQ (2003) Enzymatic synthesis of neoglycoconjugates by transglycosylation with endo-β-N-acetylglucosaminidase A. Methods Enzymol 362:64–74PubMedCrossRefGoogle Scholar
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  5. Yamamoto K, Takegawa K (1997) Transglycosylation activity of microbial endoglycosidases and its application. Trends Glycosci Glycotechnol 9:339–354Google Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Kaoru Takegawa
    • 1
  • Kenji Yamamoto
    • 2
  1. 1.Faculty of AgricultureKagawa UniversityKagawaJapan
  2. 2.Graduate School of BiostudiesKyoto UniversityKyotoJapan

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