Experimental Glycoscience


  • Naoyuki Taniguchi
  • Akemi Suzuki
  • Yukishige Ito
  • Hisashi Narimatsu
  • Toshisuke Kawasaki
  • Sumihiro Hase

Table of contents

  1. Front Matter
    Pages I-XIII
  2. Structural Analysis of Sugar Chains

    1. Front Matter
      Pages 1-1
    2. Release of Sugar Chains and Labeling

      1. Noriko Takahashi, Hirokazu Yagi, Koichi Kato
        Pages 7-11
      2. Shunji Natsuka
        Pages 12-13
      3. Makoto Ito
        Pages 18-21
      4. Shin-ichi Nakakita
        Pages 22-23
      5. Chihiro Sato
        Pages 24-27
      6. Shunji Natsuka
        Pages 28-29
      7. Mitsuhiro Kinoshita, Kazuaki Kakehi
        Pages 30-34
    3. Sequence Analysis

    4. Sugar Chain Analysis by Mass Spectrometry

    5. Analysis of the Three-dimensional Structure of Sugar Chains

      1. Masaji Ishiguro
        Pages 109-110
      2. Tsuyoshi Shirai
        Pages 111-114
    6. Analysis of Sugar-Protein Interactions

  3. Chemical Synthesis of Sugar Chains

    1. Front Matter
      Pages 143-143
    2. Chemical Synthesis of Sugar Chains

      1. Hideharu Ishida, Makoto Kiso
        Pages 147-150
      2. Toshiyuki Inazu
        Pages 151-154
      3. Yasuhiro Kajihara
        Pages 158-163
      4. Shin-ichiro Shoda
        Pages 169-172
      5. Ikuko Kakizaki, Keiichi Takagaki
        Pages 173-176
      6. Kaoru Takegawa, Kenji Yamamoto
        Pages 182-185

About this book


The aim of this book is to provide experimental protocols covering many aspects of glycobiology, glycotechnology, and chemistry: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The protocols are all self-contained descriptions of the equipment and reagents needed, followed by details of the experimental procedure.

In the post-genomic era, glycobiology is coming of age because more than half of proteins are glycosylated and the importance of sugar chains in various fields of life science research cannot be disregarded. Many scientists had not entered this area because glycobiology and glycoscience used to be considered difficult fields. This book, therefore, is presented much like a cookbook which can help scientists in fields other than glycobiology and glycoscience carry out research more easily.


Endoplasmatisches Reticulum Fluor Glycolipids Glycosyltransferase Oligosaccharid Proteoglycans biochemistry chemistry enzymes genetics glycoprotein medicine physiology proteins spectroscopy

Editors and affiliations

  • Naoyuki Taniguchi
    • 1
    • 2
  • Akemi Suzuki
    • 3
  • Yukishige Ito
    • 4
  • Hisashi Narimatsu
    • 5
  • Toshisuke Kawasaki
    • 6
  • Sumihiro Hase
    • 7
  1. 1.Department of Disease Glycomics, Research Institute for Microbial DiseasesOsaka UniversitySuita, OsakaJapan
  2. 2.Systems Glycobiology Group, Advanced Science InstituteRIKEN, WakoWako, SaitamaJapan
  3. 3.Institute of Glycotechnology, Future Science and Technology Joint Research CenterTokai UniversityHiratsuka, KanagawaJapan
  4. 4.RIKEN Discovery Research InstituteSynthetic Cellular Chemistry LaboratoryWako, SaitamaJapan
  5. 5.Research Center for Medical Glycoscience, Advanced Industrial Science and TechnologyAIST TsukubaTsukuba, IbarakiJapan
  6. 6.Research Center for GlycobiotechnologyRitsumeikan UniversityKusatsu, ShigaJapan
  7. 7.Graduate School of ScienceOsaka UniversityToyonaka, OsakaJapan

Bibliographic information

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