Glyco-Chemistry Cycle System Based on Glycosidases

  • Shin-ichiro Shoda


Carbohydrates are photosynthesized using carbon dioxide and water and are the most widely distributed organic compounds as biomass on earth. Almost all naturally occurring carbohydrates exist as glycosidic compounds; they include oligo- or polysaccharides, glycolipids, glycoproteins, and nucleosides. In the biosynthesis of these compounds, the glycosidic bonds cannot be formed by the direct dehydration reactions of saccharide units and its aglycon parts. It is necessary to activate the anomeric center of the saccharide unit by introducing an appropriate leaving group so that the anomeric carbon atom is attacked by a hydroxyl group of the aglycon part (Shoda 2001). Catalysts responsible for the glycosylation of these activated saccharides are synthases, which are classified as glycosyl transferases. After being utilized, these glycosidic compounds are finally converted to carbon dioxide and water via combustion or degradation catalyzed by glycosides from bacteria. It is, therefore, obvious that two kinds of enzymes, glycosyl transferases and glycosidases, are involved in the process of glycosylation and deglycosylation in nature, constructing a large carbon cycle system.


Glycosidic Bond Cycle System Glycosyl Transferase Glycosyl Donor Anomeric Center 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Kohri M, Kobayashi A, Noguchi M, Kawaida S, Watanabe T, Shoda S (2006) Stepwise synthesis of chitooligosaccharides through a transition-state analogue substrate catalyzed by nutants of chitinase A1 from Bacillus circulans WL-12. Holzforschung 60:485–491CrossRefGoogle Scholar
  2. Shoda S (2001) Enzymatic glycosylation. In: Fraser-Reid B, Tatsuta K, Thiem J (eds) Glycoscience, vol 2. Springer, HeidelbergGoogle Scholar
  3. Shoda S, Izumi R, Fujita M (2003) Green process in glycotechnology. Bull Soc Chem Jpn 76:1–13CrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Shin-ichiro Shoda
    • 1
  1. 1.Department of Biomolecular Engineering, Graduate School of EngineeringTohoku UniversitySendaiJapan

Personalised recommendations