Sugar Chain Synthesis by the Use of Cell Functions
For the supply of oligosaccharides as materials, extraction from natural resources, organic synthesis and enzymatic synthesis have been carried out. We developed a method of sugar chain synthesis using cell function as the fourth method (Sato et al. 2007) (Fig. 1). As the cells express a number of glycosyltransferase depending on the cell lines, which are the small factories synthesizing the sugar chain. By administering the amphiphilic alkylglycosides (saccharide primers) into the cell culture medium, the saccharide primers were glyco-sylated by cells. By biocombinatorial synthesis, i.e., combination of the saccharide primers and established cell lines, a number of oligosaccharides have been synthesized (Fig. 1).
KeywordsSensor Chip Established Cell Line HPTLC Plate Glycosyl Donor Amino Acid Conjugate
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