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Analysis of Interactions Between Carbohydrates and Proteins Using Capillary Affinity Electrophoresis

  • Mitsuhiro Kinoshita
  • Kazuaki Kakehi

Abstract

Glycosylation is one of the most important post-translational events for proteins, and plays important roles for expression of various biological events such as cell-cell interaction. However, it should be noticed that glycans attached to the protein are not a primary gene product but the product from the biosynthesis by combination of various glycosyltransferases, glycosidases, nucleotide donors, and nucleotide transporters. Thus, the glycans which attach to the core protein are highly heterogeneous in abundance, structure, and linkage position.

Keywords

Migration Time Fucose Residue Tulip Bulb Lectin Solution Constant Voltage Mode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Kinoshita M, Kakehi K (2005) Analysis of the interaction between hyaluronan and hyaluronan-binding proteins by capillary affinity electrophoresis: significance of hyaluronan molecular size on binding reaction. J Chromatogr B Analyt Technol Biomed Life Sci 816:289–295PubMedCrossRefGoogle Scholar
  2. Nakajima K, Oda Y, Kinoshita M, Kakehi K (2003) Capillary affinity electrophoresis for the screening of post-translational modification of proteins with carbohydrates. J Proteome Res 2:81–88PubMedCrossRefGoogle Scholar
  3. Nakajima K, Kinoshita M, Oda Y, Masuko T, Kaku H, Shibuya N, Kakehi K (2004) Screening method of carbohydrate-binding proteins in biological sources by capillary affinity electrophoresis and its application to determination of Tulipa gesneriana agglutinin in tulip bulbs. Glycobiology 14(9):793–804PubMedCrossRefGoogle Scholar
  4. Nakajima K, Kinoshita M, Matsushita N, Urashima T, Suzuki M, Suzuki A, Kakehi K (2006) Capillary affinity electrophoresis using lectins for the analysis of milk oligosaccharide structure and its application to bovine colostrum oligosaccharides. Anal Biochem 348(1):105–114PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Mitsuhiro Kinoshita
    • 1
  • Kazuaki Kakehi
    • 1
  1. 1.Faculty of Pharmaceutical SciencesKinki UniversityOsakaJapan

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