Skip to main content

Analysis of Interactions Between Carbohydrates and Proteins Using Capillary Affinity Electrophoresis

  • Chapter
Experimental Glycoscience

Abstract

Glycosylation is one of the most important post-translational events for proteins, and plays important roles for expression of various biological events such as cell-cell interaction. However, it should be noticed that glycans attached to the protein are not a primary gene product but the product from the biosynthesis by combination of various glycosyltransferases, glycosidases, nucleotide donors, and nucleotide transporters. Thus, the glycans which attach to the core protein are highly heterogeneous in abundance, structure, and linkage position.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 16.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Kinoshita M, Kakehi K (2005) Analysis of the interaction between hyaluronan and hyaluronan-binding proteins by capillary affinity electrophoresis: significance of hyaluronan molecular size on binding reaction. J Chromatogr B Analyt Technol Biomed Life Sci 816:289–295

    Article  PubMed  CAS  Google Scholar 

  • Nakajima K, Oda Y, Kinoshita M, Kakehi K (2003) Capillary affinity electrophoresis for the screening of post-translational modification of proteins with carbohydrates. J Proteome Res 2:81–88

    Article  PubMed  CAS  Google Scholar 

  • Nakajima K, Kinoshita M, Oda Y, Masuko T, Kaku H, Shibuya N, Kakehi K (2004) Screening method of carbohydrate-binding proteins in biological sources by capillary affinity electrophoresis and its application to determination of Tulipa gesneriana agglutinin in tulip bulbs. Glycobiology 14(9):793–804

    Article  PubMed  CAS  Google Scholar 

  • Nakajima K, Kinoshita M, Matsushita N, Urashima T, Suzuki M, Suzuki A, Kakehi K (2006) Capillary affinity electrophoresis using lectins for the analysis of milk oligosaccharide structure and its application to bovine colostrum oligosaccharides. Anal Biochem 348(1):105–114

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2008 Springer

About this chapter

Cite this chapter

Kinoshita, M., Kakehi, K. (2008). Analysis of Interactions Between Carbohydrates and Proteins Using Capillary Affinity Electrophoresis. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_34

Download citation

Publish with us

Policies and ethics