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Analyses of Sugar-Protein Interactions by NMR

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Experimental Glycoscience

Abstract

Analysis of sugar-protein interaction is an important step for elucidating the structure and function relationships of glycans. NMR spectroscopy serves an invaluable tool for this purpose because it is applicable for detecting weak sugar-protein interactions (K d ∼ mM), identifying glycotope recognized by protein, characterizing conformation of oligosaccharide bound to protein, and determining the modes of interaction between oligosaccharide and protein in solution at atomic level (Fig. 1).

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References

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Author information

Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori 3-1, Mizuho-ku, Nagoya, 467-8603, Japan

    Yoshiki Yamaguchi & Koichi Kato

Authors
  1. Yoshiki Yamaguchi
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  2. Koichi Kato
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Editor information

Editors and Affiliations

  1. Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, 1-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)

  2. Systems Glycobiology Group, Advanced Science Institute, RIKEN, Wako, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)

  3. Institute of Glycotechnology, Future Science and Technology Joint Research Center, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan

    Akemi Suzuki M.D., Ph.D. (Professor) (Professor)

  4. RIKEN Discovery Research Institute, Synthetic Cellular Chemistry Laboratory, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Yukishige Ito Ph.D. (Chief Researcher) (Chief Researcher)

  5. Research Center for Medical Glycoscience, Advanced Industrial Science and Technology, AIST Tsukuba, Central 2, Tsukuba, Ibaraki, 305-8568, Japan

    Hisashi Narimatsu M.D., Ph.D.

  6. Research Center for Glycobiotechnology, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan

    Toshisuke Kawasaki Ph.D. (Professor) (Professor)

  7. Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan

    Sumihiro Hase Ph.D. (Professor) (Professor)

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© 2008 Springer

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Yamaguchi, Y., Kato, K. (2008). Analyses of Sugar-Protein Interactions by NMR. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_32

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