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Analyses of Sugar-Protein Interactions by NMR

  • Yoshiki Yamaguchi
  • Koichi Kato

Abstract

Analysis of sugar-protein interaction is an important step for elucidating the structure and function relationships of glycans. NMR spectroscopy serves an invaluable tool for this purpose because it is applicable for detecting weak sugar-protein interactions (K d ∼ mM), identifying glycotope recognized by protein, characterizing conformation of oligosaccharide bound to protein, and determining the modes of interaction between oligosaccharide and protein in solution at atomic level (Fig. 1).

Keywords

NOESY Spectrum Residual Dipolar Coupling Chemical Shift Perturbation Saturation Transfer Difference Interproton Distance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer 2008

Authors and Affiliations

  • Yoshiki Yamaguchi
    • 1
  • Koichi Kato
    • 1
  1. 1.Graduate School of Pharmaceutical SciencesNagoya City UniversityNagoyaJapan

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