Analyses of Sugar-Protein Interactions by NMR

  • Yoshiki Yamaguchi
  • Koichi Kato


Analysis of sugar-protein interaction is an important step for elucidating the structure and function relationships of glycans. NMR spectroscopy serves an invaluable tool for this purpose because it is applicable for detecting weak sugar-protein interactions (K d ∼ mM), identifying glycotope recognized by protein, characterizing conformation of oligosaccharide bound to protein, and determining the modes of interaction between oligosaccharide and protein in solution at atomic level (Fig. 1).


NOESY Spectrum Residual Dipolar Coupling Chemical Shift Perturbation Saturation Transfer Difference Interproton Distance 
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© Springer 2008

Authors and Affiliations

  • Yoshiki Yamaguchi
    • 1
  • Koichi Kato
    • 1
  1. 1.Graduate School of Pharmaceutical SciencesNagoya City UniversityNagoyaJapan

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