Advertisement

Release of O-glycans by Chemical Methods

  • Shunji Natsuka

Abstract

Structural research of O-glycans has a smaller body of literature compared with that of N-glycans, although many physiological functions of O-glycans have been reported. This is mainly due to the lack of a suitable technique for structural analysis of trace amount of O-glycans.

Keywords

Sugar Alcohol Suitable Technique Sugar Chain Structural Research Diverse Organism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Iwase H, Ishii-Karakasa I, Fujii E, Hotta K, Hiki Y, Kobayashi Y (1992) Analysis of glycoform of Oglycan from human myeloma immunoglobulin A1 by gas-phase hydrazinolysis following pyridylamination of oligosaccharides. Anal Biochem 206:202–205PubMedCrossRefGoogle Scholar
  2. Kuraya N, Hase S (1992) Release of O-linked sugar chains from glycoproteins with anhydrous hydrazine and pyridylamination of the sugar chains with improved reaction conditions. J Biochem 112:122–126PubMedGoogle Scholar
  3. Patel T, Bruce J, Merry A, Bigge C, Wormald M, Jaques A, Parekh R (1993) Use of hydrazine to release in intact and unreduced form both N-and O-linked oligosaccharides from glycoproteins. Biochemistry 32:679–693PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Shunji Natsuka
    • 1
  1. 1.Department of Chemistry, Graduate School of ScienceOsaka UniversityToyonaka, OsakaJapan

Personalised recommendations