Release of O-glycans by Chemical Methods

  • Shunji Natsuka


Structural research of O-glycans has a smaller body of literature compared with that of N-glycans, although many physiological functions of O-glycans have been reported. This is mainly due to the lack of a suitable technique for structural analysis of trace amount of O-glycans.


Sugar Alcohol Suitable Technique Sugar Chain Structural Research Diverse Organism 


  1. Iwase H, Ishii-Karakasa I, Fujii E, Hotta K, Hiki Y, Kobayashi Y (1992) Analysis of glycoform of Oglycan from human myeloma immunoglobulin A1 by gas-phase hydrazinolysis following pyridylamination of oligosaccharides. Anal Biochem 206:202–205PubMedCrossRefGoogle Scholar
  2. Kuraya N, Hase S (1992) Release of O-linked sugar chains from glycoproteins with anhydrous hydrazine and pyridylamination of the sugar chains with improved reaction conditions. J Biochem 112:122–126PubMedGoogle Scholar
  3. Patel T, Bruce J, Merry A, Bigge C, Wormald M, Jaques A, Parekh R (1993) Use of hydrazine to release in intact and unreduced form both N-and O-linked oligosaccharides from glycoproteins. Biochemistry 32:679–693PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Shunji Natsuka
    • 1
  1. 1.Department of Chemistry, Graduate School of ScienceOsaka UniversityToyonaka, OsakaJapan

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