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Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry

  • Minoru Suzuki

Abstract

For the analysis of oligosaccharides derived from glycosphingolipids, a method involving pyridylamino (PA) derivatization, normal- and reversed-phase HPLC with fluorescent detection (Hase 1994) after treatment with endoglucoceramidase (Ito and Yamagata 1986) is widely used. In order to characterize structures of the PA-oligosaccharides, matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS), which has the capability to perform MS n analysis, has been successfully used (Suzuki et al. 2005).

Keywords

Linkage Isomer Spectrum Select Acidic Glycosphingolipids Expression Laboratory Oligosaccharide Isomer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Hase S (1994) High-performance liquid chromatography of pyridyl-aminated saccharides. Methods Enzymol 230:225–237PubMedCrossRefGoogle Scholar
  2. Ito M, Yamagata T (1986) A novel glycosphingolipid degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids. J Biol Chem 261:14278–14282PubMedGoogle Scholar
  3. Suzuki Y, Suzuki M, Ito E, Ishii H, Miseki K, Suzuki A (2005) Convenient and rapid analysis of linkage isomers of fucose-containing oligosaccharides by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry. Glycoconj J 22:427–431PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Minoru Suzuki
    • 1
  1. 1.Sphingolipid Expression LaboratoryRIKEN Frontier Research SystemWako, SaitamaJapan

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