Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry
For the analysis of oligosaccharides derived from glycosphingolipids, a method involving pyridylamino (PA) derivatization, normal- and reversed-phase HPLC with fluorescent detection (Hase 1994) after treatment with endoglucoceramidase (Ito and Yamagata 1986) is widely used. In order to characterize structures of the PA-oligosaccharides, matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS), which has the capability to perform MS n analysis, has been successfully used (Suzuki et al. 2005).
KeywordsLinkage Isomer Spectrum Select Acidic Glycosphingolipids Expression Laboratory Oligosaccharide Isomer
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- Suzuki Y, Suzuki M, Ito E, Ishii H, Miseki K, Suzuki A (2005) Convenient and rapid analysis of linkage isomers of fucose-containing oligosaccharides by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry. Glycoconj J 22:427–431PubMedCrossRefGoogle Scholar