Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry

Suzuki, Minoru

doi:10.1007/978-4-431-77924-7_19

Minoru Suzuki⁸

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Abstract

For the analysis of oligosaccharides derived from glycosphingolipids, a method involving pyridylamino (PA) derivatization, normal- and reversed-phase HPLC with fluorescent detection (Hase 1994) after treatment with endoglucoceramidase (Ito and Yamagata 1986) is widely used. In order to characterize structures of the PA-oligosaccharides, matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS), which has the capability to perform MSⁿ analysis, has been successfully used (Suzuki et al. 2005).

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References

Hase S (1994) High-performance liquid chromatography of pyridyl-aminated saccharides. Methods Enzymol 230:225–237
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Ito M, Yamagata T (1986) A novel glycosphingolipid degrading enzyme cleaves the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids. J Biol Chem 261:14278–14282
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Authors and Affiliations

Sphingolipid Expression Laboratory, RIKEN Frontier Research System, 2-1 Hirosawa, Wako, Saitama, 351-1098, Japan
Minoru Suzuki

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Minoru Suzuki
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Editors and Affiliations

Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, 1-1 Yamadaoka, Suita, Osaka, 565-0871, Japan
Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)
Systems Glycobiology Group, Advanced Science Institute, RIKEN, Wako, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)
Institute of Glycotechnology, Future Science and Technology Joint Research Center, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan
Akemi Suzuki M.D., Ph.D. (Professor) (Professor)
RIKEN Discovery Research Institute, Synthetic Cellular Chemistry Laboratory, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan
Yukishige Ito Ph.D. (Chief Researcher) (Chief Researcher)
Research Center for Medical Glycoscience, Advanced Industrial Science and Technology, AIST Tsukuba, Central 2, Tsukuba, Ibaraki, 305-8568, Japan
Hisashi Narimatsu M.D., Ph.D.
Research Center for Glycobiotechnology, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan
Toshisuke Kawasaki Ph.D. (Professor) (Professor)
Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan
Sumihiro Hase Ph.D. (Professor) (Professor)

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Suzuki, M. (2008). Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry. In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_19

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DOI: https://doi.org/10.1007/978-4-431-77924-7_19
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-77923-0
Online ISBN: 978-4-431-77924-7
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