Microsequencing of Functional Chondroitin Sulfate Oligosaccharides

  • Kazuyuki Sugahara
  • Shuhei Yamada


Accumulating evidence implies that chondroitin sulfate (CS)/dermatan sulfate (DS) has a wide range of biological activities, which result from the ability to interact with various proteins, such as growth factors, cytokines, and neurotrophic factors, thereby causing their activation, inactivation, or stabilization. CS/DS chains are linear polysaccharides composed of the various disaccharide units shown in Fig. 1 (Nandini and Sugahara 2006). Functional domains with distinct sequences, which are recognized by specific protein ligands, are formed by combinations of these disaccharide units. Here, methods for the microsequencing of CS oligosaccharides with specific biological activities are described.


Chondroitin Sulfate Paper Chromatography Dermatan Sulfate Internal Position Disaccharide Unit 
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Copyright information

© Springer 2008

Authors and Affiliations

  • Kazuyuki Sugahara
    • 1
  • Shuhei Yamada
    • 1
  1. 1.Laboratory of Proteoglycan Signaling and Therapeutics, Graduate School of Life ScienceSapporoJapan

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