Microsequencing of Functional Chondroitin Sulfate Oligosaccharides
Accumulating evidence implies that chondroitin sulfate (CS)/dermatan sulfate (DS) has a wide range of biological activities, which result from the ability to interact with various proteins, such as growth factors, cytokines, and neurotrophic factors, thereby causing their activation, inactivation, or stabilization. CS/DS chains are linear polysaccharides composed of the various disaccharide units shown in Fig. 1 (Nandini and Sugahara 2006). Functional domains with distinct sequences, which are recognized by specific protein ligands, are formed by combinations of these disaccharide units. Here, methods for the microsequencing of CS oligosaccharides with specific biological activities are described.
KeywordsChondroitin Sulfate Paper Chromatography Dermatan Sulfate Internal Position Disaccharide Unit
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- Deepa SS, Kalayanamitra K, Ito Y, Kongtawelert P, Fukui S, Yamada S, Mikami T, Sugahara K (2007a) Novel sulfated octa-and decasaccharides from squid cartilage chondroitin sulfate-E: sequencing and their application for determination of the epitope structure of monoclonal antibody MO-225. Biochemistry 46:2453–2465PubMedCrossRefGoogle Scholar
- Kinoshita A, Sugahara K (1999) Microanalysis of glycosaminoglycan-derived oligosaccharides labeled with the fluorophore 2-aminobenzamide by high-performance liquid chromatography: application to disaccharide composition analysis and exo-sequencing of oligosaccharides. Anal Biochem 269:367–378PubMedCrossRefGoogle Scholar
- Nandini CD, Sugahara K (2006) Role of the sulfation pattern of chondroitin sulfate in its biological activities in the binding of growth factors. In: Volpi N (ed) Advances in pharmacology, vol 53. Elsevier, UK, pp 253–279Google Scholar