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DNA Microarray in Glycobiology

  • Harumi Yamamoto
  • Hiromu Takematsu
  • Yasunori Kozutsumi

Abstract

DNA microarray analysis enables us to provide a considerable amount of information on glyco-chain expression (Brazma et al. 2001; Ide et al. 2006). DNA microarray experiments are developed to obtain overall transcriptome of cells using DNAs spotted on a slide glass as probes and fluorescently labeled DNAs derived from mRNA samples. DNA microarray experiments require high-quality RNA. Thus, it is advisable to prepare RNA as intact as possible. The quality of RNA is usually assessed by the intactness of the peaks and the lack of degradation of RNA by capillary electrophoresis. This step is essential for the reliable final readout of microarray experiments. Following is the normal procedure operated in our laboratory for the DNA microarray experiments using RIKEN human and mouse glyco-chain-related DNA microarrays version 2 (human version: GEO platform GPL 3465 http://www.ncbi.nlm.nih.gov/geo/), which were designed by us and spotted by Takara Bio Inc.

Keywords

Sialic Acid Microarray Experiment Induce Adhesion Molecule Sialyltransferase Gene Germinal Center Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer 2008

Authors and Affiliations

  • Harumi Yamamoto
    • 1
  • Hiromu Takematsu
    • 2
    • 3
  • Yasunori Kozutsumi
    • 1
    • 2
    • 3
  1. 1.Supra-biomolecular System Research GroupRIKEN Frontier Research SystemWako, SaitamaJapan
  2. 2.Laboratory of Membrane Biochemistry and Biophysics, Graduate School of BiostudiesKyoto UniversityKyotoJapan
  3. 3.CRESTJSTKawaguchi, SaitamaJapan

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