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Molecular Analysis of the Retinoblastoma (Rb) Gene in Human Colorectal Carcinomas

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Hereditary Colorectal Cancer

Abstract

We have examined the structure and expression of the Rb gene on chromosome 13 in matched pairs of normal and malignant colonic tissues from 68 patients. Northern blots showed a normal size 4.7 kb transcript in the 33 normal mucosal tissues and 38 colorectal carcinomas examined. However, increased expression of the Rb gene (2-5 fold) was detected in approximately 70% of colorectal carcinomas relative to normal colonic mucosa. The remaining malignant tissues showed an equal level of Rb specific RNA as compared to normal colonic mucosa and none showed a decrease in hybridization signal. Southern blot analysis of DNA from 45 colorectal carcinomas showed an increase in hydridization intensity (at least two fold) in 20 tumors in comparison to normal mucosa. These results coupled with previous reports of nonrandom chromosome 13 gains in approximately 50% of colorectal carcinomas suggest that an increase in Rb gene copy number occurs frequently in these tumors. Southern blot analysis also shows that none of the colorectal cancers examined had allelic loss at the Rb locus. These results suggest that the Rb gene is not lost or transcriptionally inactivated in colorectal cancers.

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Authors
  1. Rajalakshmi Gope
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  2. Mark A. Christensen
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  3. Alan Thorson
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  4. Henry T. Lynch
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  5. Thomas Smyrk
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  6. Mohan L. Gope
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  7. Bruce M. Boman
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Editor information

Editors and Affiliations

  1. Second Department of Surgery, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo, 663, Japan

    Joji Utsunomiya M. D. (Professor and Chairman) (Professor and Chairman)

  2. Department of Preventive Medicine, Creighton University School of Medicine, Hereditary Cancer Center, California at 24th Street, Omaha, NE, 68178, USA

    Henry T. Lynch M. D. (Professor and Chairman) (Professor and Chairman)

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© 1990 Springer Japan

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Gope, R. et al. (1990). Molecular Analysis of the Retinoblastoma (Rb) Gene in Human Colorectal Carcinomas. In: Utsunomiya, J., Lynch, H.T. (eds) Hereditary Colorectal Cancer. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68337-7_73

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  • DOI: https://doi.org/10.1007/978-4-431-68337-7_73

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-68339-1

  • Online ISBN: 978-4-431-68337-7

  • eBook Packages: Springer Book Archive

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