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Histochemical Analyses of Living Mouse Liver Under Different Hemodynamic Conditions

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In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

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Abstract

Morphology and molecular distribution in animal liver tissues were examined using “in vivo cryotechnique” (IVCT) and compared with findings obtained with other conventional tissue preparation methods. Open sinusoids with flowing erythrocytes were clearly observed under normal blood circulation, and blood congestion and sinusoidal collapse were apparent in liver under ischemic or heart-arrest conditions. Sinusoidal collapse was also apparent with conventional tissue preparation methods, including immersion-fixation and quick-freezing (QF) methods of resected tissues, while perfusion-fixation artificially dilated the sinusoidal cavities. The IVCT and QF method well maintained immunoreactivity of soluble serum proteins including albumin and immunoglobulins as well as glycogen in hepatocytes detected by periodic acid-Schiff (PAS) staining. Rapid molecular translocation of serum proteins into hepatocytes was immunohistochemically observed after tissue resection with the QF method. IVCT was useful to examine dynamic morphology under different hemodynamic states and also immunodistribution of soluble components in living mouse livers under physiological and pathological states with higher time resolution.

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Authors and Affiliations

  1. Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo City, Yamanashi, 409-3898, Japan

    Nobuhiko Ohno M.D., Ph.D., Nobuo Terada & Shinichi Ohno

  2. Division of Health Sciences, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto City, Nagano, 390-8621, Japan

    Nobuo Terada

Authors
  1. Nobuhiko Ohno M.D., Ph.D.
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  2. Nobuo Terada
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  3. Shinichi Ohno
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Correspondence to Nobuhiko Ohno M.D., Ph.D. .

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Editors and Affiliations

  1. Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

    Shinichi Ohno

  2. Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan

    Nobuhiko Ohno

  3. Division of Health Science, Shinshu University Graduate School of Medicine, Nagano, Japan

    Nobuo Terada

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Ohno, N., Terada, N., Ohno, S. (2016). Histochemical Analyses of Living Mouse Liver Under Different Hemodynamic Conditions. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_5

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  • DOI: https://doi.org/10.1007/978-4-431-55723-4_5

  • Publisher Name: Springer, Tokyo

  • Print ISBN: 978-4-431-55722-7

  • Online ISBN: 978-4-431-55723-4

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