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In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs

  • Shinichi Ohno
  • Nobuhiko Ohno
  • Nobuo Terada

Table of contents

  1. Front Matter
    Pages i-xi
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Shinichi Ohno
      Pages 3-5
    3. Shinichi Ohno
      Pages 11-14
    4. Shinichi Ohno
      Pages 15-16
  3. Application of ‘IVCT’ to Various Mouse Organs

    1. Front Matter
      Pages 17-17
    2. Nobuhiko Ohno, Nobuo Terada, Shinichi Ohno
      Pages 19-23
    3. Yurika Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 25-28
    4. Satoshi Shimo, Sei Saitoh, Yurika Saitoh, Nobuhiko Ohno, Shinichi Ohno
      Pages 33-38
    5. Jiaorong Chen, Nobuo Terada, Yurika Saitoh, Zheng Huang, Nobuhiko Ohno, Shinichi Ohno
      Pages 39-44
    6. Yuqin Bai, Bao Wu, Nobuo Terada, Nobuhiko Ohno, Yurika Saitoh, Shinichi Ohno
      Pages 45-49
    7. Sei Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 51-56
    8. Liye Shi, Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Shinichi Ohno
      Pages 67-76
    9. Yurika Saitoh, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 77-81
    10. Shinichi Ohno, Nobuo Terada, Yasuhisa Fujii
      Pages 83-86
    11. Yuqin Bai, Bao Wu, Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Sei Saitoh et al.
      Pages 87-92
    12. Bao Wu, Nobuhiko Ohno, Yurika Saitoh, Yuqin Bai, Zheng Huang, Nobuo Terada et al.
      Pages 93-99
    13. Zilong Li, Nobuhiko Ohno, Nobuo Terada, Shinichi Ohno
      Pages 101-104
    14. Zilong Li, Nobuo Terada, Nobuhiko Ohno, Shinichi Ohno
      Pages 105-108
    15. Zilong Li, Xiaoyue Zhai, Nobuhiko Ohno, Nobuo Terada, Shinichi Ohno
      Pages 109-113
    16. Nobuhiko Ohno, Daoyuan Zhou, Nobuo Terada, Shinichi Ohno
      Pages 121-125
    17. Eri Kawashima, Yurika Saitoh, Nobuo Terada, Nobuhiko Ohno, Kiyoko Inui, Ashio Yoshimura et al.
      Pages 127-133
    18. Sei Saitoh, Nobuo Terada, Nobuhiko Ohno, Yurika Saitoh, Shinichi Ohno
      Pages 135-140
    19. Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Yasuhisa Fujii, Shinichi Ohno
      Pages 145-147
    20. Nobuhiko Ohno, Hong Zhou, Nobuo Terada, Shinichi Ohno
      Pages 155-160
    21. Zheng Huang, Nobuhiko Ohno, Nobuo Terada, Yurika Saitoh, Jiaorong Chen, Shinichi Ohno
      Pages 165-170
    22. Nobuhiko Ohno, Nobuo Terada, Shinichi Ohno
      Pages 175-178
    23. Nobuhiko Ohno, Nobuo Terada, Yurika Saitoh, Shinichi Ohno
      Pages 179-183
  4. Further Preparation After ‘IVCT’

    1. Front Matter
      Pages 197-197
    2. Shinichi Ohno
      Pages 199-202
  5. Comments on Advantages of ‘IVCT’

    1. Front Matter
      Pages 203-203
    2. Shinichi Ohno, Nobuo Terada, Yasuhisa Fujii, Nobuhiko Ohno
      Pages 211-214
  6. Direct Detection of Molecules by Raman Microscopy

    1. Front Matter
      Pages 223-223
    2. Nobuo Terada, Yurika Saitoh, Nobuhiko Ohno, Yasuhisa Fujii, Shinichi Ohno
      Pages 225-227

About this book

Introduction

This book focuses on actual morphofunctional findings of cells and tissues in living animal organs. Medical and biological scientists need to know the real in vivo morphology and immunolocalization of the molecular components in living animal organs. Recently, the live imaging of cells and tissues of animals with fluorescence-labeled proteins by gene manipulation has become more and more popular in biological fields. Current research, meanwhile, has revealed that immunohistochemical or morphological studies exclusively depend on living animal organs. The cryotechnique is one of the most useful tools for immunohistochemistry and bioimaging of animal organs. This book describes the epoch-making cryotechnique originally developed by the editors. The book also makes the management of living animal morphology more accessible not only for biomedical researchers but also for clinical doctors, providing a valuable resource work on the current perspectives of in vivo morphology.

Keywords

Cryostat section Immunocytochemistry Live imaging Living Morphology Microscope Analysis

Editors and affiliations

  • Shinichi Ohno
    • 1
  • Nobuhiko Ohno
    • 2
  • Nobuo Terada
    • 3
  1. 1.Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and EngineeringUniversity of YamanashiYamanashiJapan
  2. 2.Department of Anatomy and Molecular Histology Interdisciplinary Graduate School of Medicine and EngineeringUniversity of YamanashiYamanashiJapan
  3. 3.Division of Health ScienceShinshu University Graduate School of MedicineNaganoJapan

Bibliographic information

  • DOI https://doi.org/10.1007/978-4-431-55723-4
  • Copyright Information Springer Japan 2016
  • Publisher Name Springer, Tokyo
  • eBook Packages Medicine
  • Print ISBN 978-4-431-55722-7
  • Online ISBN 978-4-431-55723-4
  • Buy this book on publisher's site
Industry Sectors
Pharma
Health & Hospitals
Biotechnology