Abstract
Blood-follicle barrier (BFB) in ovarian follicles is the molecular sieve selective for size and charge. By utilizing the “in vivo cryotechnique” (IVCT), ovarian structures responsible for the BFB were analyzed during development of follicles under physiological conditions. Immunoreactivity of mouse serum proteins was better preserved with IVCT compared with other conventional methods. Strong immunoreactivity of albumin was detected in blood vessels, interstitium, and developing follicles. There was a clear alteration of the immunostaining intensity of IgG1 between inside and outside of the follicular basement membranes. Immunoreactivity of IgM was significantly changed between inside and outside of the vascular endothelial cells. These results suggest that permselectivity of BFB for soluble proteins with intermediate molecular weights is dependent on the follicular basement membrane and the vascular endothelial cells could play significant roles in the permselectivity for soluble proteins with high molecular weight.
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Ohno, N., Zhou, H., Terada, N., Ohno, S. (2016). Involvement of Follicular Basement Membrane and Vascular Endothelium in Blood-Follicle Barrier Formation of Mice. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_28
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DOI: https://doi.org/10.1007/978-4-431-55723-4_28
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