Abstract
Dynamin (Dyn) is a GTPase that promotes endocytic processes through scission of cell membranes. In this chapter, we present the contribution of Dyn2 to the structural integrity of discoid vesicles (DVs) and to the endocytic activity of urinary bladder umbrella cells (UCs) which have capacity to control bladder volume. Dyn2 was abundantly expressed in UCs, especially near the apical cytoplasmic regions. By immunoelectron microscopy, Dyn2 was found on and around DV membranes in UCs. Ultrastructural analysis with a quick-freezing and deep-etching method revealed the existence of distinct Dyn2-bound microfilaments in close association with DV membranes. Treatment of bladders with a Dyn-GTPase inhibitor, dynasore, markedly reduced the number of DVs in UCs. In infected UCs, Escherichia coli was encased in compartments enriched in Dyn2, and treatment of bladders with dynasore inhibits E. coli invasion of UCs.
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Terada, N., Saitoh, Y., Ohno, N., Fujii, Y., Ohno, S. (2016). Function of Dynamin-2 in the Formation of Discoid Vesicles in Urinary Bladder Umbrella Cells. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_27
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DOI: https://doi.org/10.1007/978-4-431-55723-4_27
Publisher Name: Springer, Tokyo
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