Abstract
The mechanisms of serum protein passage through glomerular capillary loops are unknown to us yet. We have visualized topographical changes of the serum proteins by “in vivo cryotechnique” in combination with immunohistochemistry. Albumin and immunoglobulin G, Ig kappa light chain, and IgG1 heavy chain were mainly immunolocalized in GCL, but not colocalized with zonula occludens-1 (ZO-1) under normotensive condition. Albumin and kappa light chain were immunolocalized in Bowman’s space under heart-arrest condition and in quick-frozen fresh tissues. However, they were more clearly immunolocalized along basement membranes and in Bowman’s space under acute hypertensive condition, indicating their increased passage through GCL. IgG was also more clearly localized in mesangial areas (MA) under acute hypertension, compared with that under the normotensive or heart-arrest condition. This study is the first direct visualization for glomerular passage of serum proteins under abnormal hemodynamic conditions by the “in vivo cryotechnique,” which is a useful protocol for morphofunctional examination of living mouse GCL and immunohistochemical analyses of dynamically changing proteins.
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References
Ohno S, Baba T, Terada N, Fujii Y, Ueda H (1996) Cell biology of kidney glomerulus. Int Rev Cytol 166:181–230
Ohno S, Terada N, Fujii Y, Ueda H, Takayama I (1996) Dynamic structure of glomerular capillary loop as revealed by an in vivo cryotechnique. Virchows Arch 427:519–527
Olivetti G, Kithier K, Giacomelli F, Wiener J (1981) Glomerular permeability to endogenous proteins in the rat: effects of acute hypertension. Lab Invest 44:127–137
Remuzzi G, Bertani T (1998) Pathophysiology of progressive nephropathies. N Engl J Med 339:1448–1456
Li Z, Terada N, Ohno N, Ohno S (2005) Immunohistochemical analyses on albumin and immunoglobulin in acute hypertensive mouse kidneys by “in vivo cryotechnique”. Histol Histopathol 20:807–816
Gansevoort RT, Navis GJ, Wapstra FH, de Jong PE, de Zeeuw D (1997) Proteinuria and progression of renal disease: therapeutic implications. Curr Opin Nephrol Hypertens 6:133–140
Jerums G, Panagiotopoulos S, Tsalamandris C, Allen TJ, Gilbert RE, Comper WD (1997) Why is proteinuria such an important risk factor for progression in clinical trials? Kidney Int Suppl 63:S87–S92
Remuzzi G (1995) Abnormal protein traffic through the glomerular barrier induces proximal tubular cell dysfunction and causes renal injury. Curr Opin Nephrol Hypertens 4:339–342
Schreiner GF (1995) Renal toxicity of albumin and other lipoproteins. Curr Opin Nephrol Hypertens 4:369–373
Thomas ME, Brunskill NJ, Harris KP, Bailey E, Pringle JH, Furness PN, Walls J (1999) Proteinuria induces tubular cell turnover: a potential mechanism for tubular atrophy. Kidney Int 55:890–898
Reiser J, Kriz W, Kretzler M, Mundel P (2000) The glomerular slit diaphragm is a modified adherens junction. J Am Soc Nephrol 11:1–8
Rico M, Mukherjee A, Konieczkowski M, Bruggeman LA, Miller RT, Khan S, Schelling JR, Sedor JR (2005) WT1-interacting protein and ZO-1 translocate into podocyte nuclei after puromycin aminonucleoside treatment. Am J Physiol Renal Physiol 289:F431–F441
Fujigaki Y, Nagase M, Kobayasi S, Hidaka S, Shimomura M, Hishida A (1993) Intra-GBM site of the functional filtration barrier for endogenous proteins in rats. Kidney Int 43:567–574
Yu Y, Leng CG, Kato Y, Terada N, Fujii Y, Ohno S (1998) Ultrastructural study of anionic sites in glomerular basement membranes at different perfusion pressures by quick-freezing and deep-etching method. Nephron 78:88–95
Yu Y, Leng CG, Terada N, Ohno S (1998) Scanning electron microscopic study of the renal glomerulus by an in vivo cryotechnique combined with freeze-substitution. J Anat 192:595–603
Ohno N, Terada N, Fujii Y, Baba T, Ohno S (2004) “In vivo cryotechnique” for paradigm shift to “living morphology” of animal organs. Biomed Rev 15:1–19
Terada N, Ohno N, Li Z, Fujii Y, Baba T, Ohno S (2006) Application of in vivo cryotechnique to the examination of cells and tissues in living animal organs. Histol Histopathol 21(3):265–272
Ohno N, Terada N, Ohno S (2004) Advanced application of the in vivo cryotechnique to immunohistochemistry for animal organs. Acta Histochem Cytochem 37:357–364
Ohno N, Terada N, Murata S, Katoh R, Ohno S (2005) Application of cryotechniques with freeze-substitution for the immunohistochemical demonstration of intranuclear pCREB and chromosome territory. J Histochem Cytochem 53:55–62
Bijl M, Dijstelbloem HM, Oost WW, Bootsma H, Derksen RH, Aten J, Limburg PC, Kallenberg CG (2002) IgG subclass distribution of autoantibodies differs between renal and extra-renal relapses in patients with systemic lupus erythematosus. Rheumatology 41:62–67
Imai H, Hamai K, Komatsuda A, Ohtani H, Miura AB (1997) IgG subclasses in patients with membranoproliferative glomerulonephritis, membranous nephropathy, and lupus nephritis. Kidney Int 51:270–276
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Li, Z., Ohno, N., Terada, N., Ohno, S. (2016). Immunolocalization of Serum Proteins in Living Mouse Glomeruli Under Various Hemodynamic Conditions. In: Ohno, S., Ohno, N., Terada, N. (eds) In Vivo Cryotechnique in Biomedical Research and Application for Bioimaging of Living Animal Organs. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55723-4_19
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DOI: https://doi.org/10.1007/978-4-431-55723-4_19
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