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Genetic System of Organohalide-Respiring Bacteria

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Biodegradative Bacteria

Abstract

Organohalide-respiring bacteria (OHRB) utilize halogenated organic compounds as terminal electron acceptors and are considered to be significantly important from both viewpoints of bioremediation and natural halogen cycle. Growth-linked bioremediation using OHRB has been successfully applied to removal of chlorinated solvents, e.g., tetrachloroethene is successively converted to trichloroethene, dichloroethenes, vinyl chloride, and nontoxic ethene. From OHRB, versatile reductive dehalogenases (RDases), which catalyze the reductive dehalogenation reaction, were purified and their corresponding genes have been identified. In this chapter, we present an overview of current understanding of organohalide respiration, showing the RDase genes and their associated genes are highly conserved in phylogenetically diverse OHRB.

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Abbreviations

3Cl4OHPA:

3-Chloro-4-hydroxyphenylacetate

4-OHPA:

4-Hydroxyphenylacetate

CARD-FISH:

Catalyzed reporter deposition-fluorescent in situ hybridization

CD:

Carbon dichloride

CF:

Chloroform

cis-DCE:

cis-1,2-dichloroethene

CP:

Chlorophenol

DMSO:

Dimethyl sulfoxide

EPR:

Electron paramagnetic resonance

ETH:

Ethene

IS:

Insertion sequence

OHRB:

Organohalide-respiring bacteria

PCBs:

Polychlorinated biphenyls

PCE:

Tetrachloroethene

RDase:

Reductive dehalogenase

Tat:

Twin-arginine translocation

TCB:

Trichlorobenzene

TCE:

Trichloroethene

TeCB:

Tetrachlorobenzene

trans-DCE:

trans-1,2-dichloroethene

VC:

Vinyl chloride

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Acknowledgment

The research group of K.F. is supported by grants from the Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Kyushu University, 6-10-1, Hakozaki, Fukuoka, 812-8581, Japan

    Taiki Futagami & Masatoshi Goto

  2. Department of Food and Bioscience, Faculty of Food Science and Nutrition, Beppu University, Kitaishigaki 82, Beppu, Oita, 874–8501, Japan

    Kensuke Furukawa

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  1. Taiki Futagami
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  2. Masatoshi Goto
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  3. Kensuke Furukawa
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Correspondence to Taiki Futagami .

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  1. Biotechnology Research Center, The University of Tokyo, Tokyo, Japan

    Hideaki Nojiri

  2. Graduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan

    Masataka Tsuda

  3. Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan

    Masao Fukuda

  4. Bioproduction Research Institute, Nat'l Inst of Adv Industr Sci and Tech, Sapporo, Hokkaido, Japan

    Yoichi Kamagata

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Futagami, T., Goto, M., Furukawa, K. (2014). Genetic System of Organohalide-Respiring Bacteria. In: Nojiri, H., Tsuda, M., Fukuda, M., Kamagata, Y. (eds) Biodegradative Bacteria. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54520-0_4

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