Short History of Electroporation for the Study of Developmental Biology
Electroporation at high voltage has been been applied for transformation of bacteria. Since it damages the tissue of higher organisms, it has been limited to bac teria trans formation. Nevertheless, electroporation in higher organisms has been carried out for the transfer of the drugs for treatment of cancer; bleomycin was successfully applied to hepatocellular carcinoma in the Donryu rat (Okino and Mohri, 1987). Mr. Hayakawa (Nepa Gene; Ichikawa, Japan) supplied the electroporator and helped to optimize the condition of electroporation with the BTX electropo rator, and Mr. Imada (Unique Medical-Imada, Natori, Japan) designed and prepared electrodes.
Suggested by successful drug delivery by electroporation, gene transfer by electroporation has been attempted by high voltage. First, transfection of gus gene (beta-glucronidase) by electroporation into plant material was carried out (Hayakawa, personal communication). Then, transfection of lacZ in brain tumor was tried. Electroporation at 300 V/0.5 cm was carried out after injection of expres sion vector into the carotid artery, and the expression of lacZ was limited to the tumor site, which received an electric charge (Nishi et al., 1996).
KeywordsGene Transfer Chick Embryo Chicken Embryo Gene Transfer System Increase Cell Membrane Permeability
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