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  • Book
  • © 2009

Electroporation and Sonoporation in Developmental Biology

Editors:

  1. Harukazu Nakamura

    1. Graduate School of Life Sciences/Institute of Development, Aging & Cancer, Tohoku University, Aoba-ku, Japan

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Table of contents (27 chapters)

  1. Front Matter

    Pages i-xxix
    PDF

  2. Electroporation

    5. In Ovo Electroporation for Targeting the Somitic Mesoderm

      • Emi Ohata, Yoshiko Takahashi
      Pages 37-42

    6. Method of Electroporation for the Early Chick Embryo

      • Jun Hatakeyama, Kenji Shimamura
      Pages 43-53

    7. Enhancer Analysis

      • Masanori Uchikawa, Tatsuya Takemoto
      Pages 55-71

    9. Electroporation into the Limb: Beyond Misexpression

      • Takayuki Suzuki, Toshihiko Ogura
      Pages 85-96

    10. Retinal Fiber Tracing by In Ovo Electroporation

      • Hidekiyo Harada, Harukazu Nakamura
      Pages 97-104

    13. Electroporation into Cultured Mammalian Embryos

      • Tadashi Nomura, Masanori Takahashi, Noriko Osumi
      Pages 129-141

    15. Practical Application of Microelectroporation into Developing Mouse Brain

      • Tomomi Shimogori, Masaharu Ogawa
      Pages 153-167

    16. Single Cell Electroporation Method for Mammalian CNS Neurons in Organotypic Slice Cultures

      • Naofumi Uesaka, Yasufumi Hayano, Akito Yamada, Nobuhiko Yamamoto
      Pages 169-177

    18. Gene Transfer into Mouse Haemogenic Sites, as a Mean to Functionally Approach the Control of Mesoderm Determination Towards a Haematopoietic Fate

      • Anna-Lila Kaushik, Sébastien J. D. Giroux, Michèle Klaine, Ali Jalil, Yann Lécluse, Isabelle Godin
      Pages 201-217

    19. Electroporation of Embryonic Kidney Explants

      • Nicholas Haddad, Daniel Houle, Indra R. Gupta
      Pages 219-227
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About this book

In ovo electroporation is an epoch-making achievement in the study of developmental biology. With this method, experiments can be carried out in gain and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of interest. It is now applied to mice, aquatic animals, and even to plants for the study of developmental biology and for other purposes. In this book, the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced.

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Keywords

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Editors and Affiliations

  • Graduate School of Life Sciences/Institute of Development, Aging & Cancer, Tohoku University, Aoba-ku, Japan

    Harukazu Nakamura

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Bibliographic Information

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Buy it now

eBook USD 119.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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