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To Establish the Regeneration System of Sweet Sorghum Immature Embryos

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Advances in Applied Biotechnology

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 333))

Abstract

Sweet sorghum is an important forage and energy crop. In this experiment, sweet sorghum immature embryos were taken to be explants, through inducting and regenerating for sweet sorghum callus of different genotypes, to obtain the best culture medium formula and create appropriate conditions for establishing efficient regeneration system. The result showed that, for callus induction, the response of immature embryos varied with the genotype, but the medium is not. Of these, Xinliang 52 showed the highest frequency of callus induction under appropriate culture conditions. As regards regenerable callus formation, the response of callus varied with the genotype and medium. 07-27 regeneration occurred at high frequencies when MSR and MBR were added in the regeneration medium. And there is nothing on NBI medium for the whole genotypes. So NBI is not suitable for callus regeneration culture.

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Acknowledgments

This work was supported by the innovative talents training plan of the young and middle-aged backbone of Tianjin and the innovation team project of genetic improvement of the crop quality and resistance of Tianjin, for which the authors are thankful.

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Authors and Affiliations

  1. Agriculture Resource and Environment College, Tianjin Agricultural University, Tianjin, China

    Xiaomu Chen, Oujing Li, Lili Shi, Xianhua Wu, Boxian Xia & Zhongyou Pei

Authors
  1. Xiaomu Chen
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  2. Oujing Li
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  3. Lili Shi
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  4. Xianhua Wu
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  5. Boxian Xia
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  6. Zhongyou Pei
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Corresponding author

Correspondence to Zhongyou Pei .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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Chen, X., Li, O., Shi, L., Wu, X., Xia, B., Pei, Z. (2015). To Establish the Regeneration System of Sweet Sorghum Immature Embryos. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_9

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