Abstract
Microcystis has been regarded as the most common cause of water bloom. The spatial and temporal distribution of Microcystis provides a method of bloom warning. Microcystis novacekii was the dominant cyanobacteria species in Yuqiao reservoir in recent years. Seven sampling stations were set and the phytoplankton were collected in both July and October. Comparing morphological observation with the molecular biological method, which is based on 16S rDNA sequencing, the recognizing research of M. novacekii was conducted. The results showed that the average biomass of M. novacekii was 0.92 ± 0.23 mg/L; cell density was 1833.48 ± 457.70 × 104/L affected by light, temperature, nutrients, and other environmental factors. In July, the maximum biomass was 2.21 mg/L at Center station and the minimum was 0.83 mg/L at the same site; in October, the maximum was 0.18 mg/L at intake site, the minimum was 0.09 mg/L at Center West. The significant seasonal fluctuation meant that M. novacekii favored high temperature, which is the same habit with universal Microcystis algae. 30 clones of M. novacekii were collected and amplified the 16S rDNA fragments. Results proved that the molecular identification of Microcystis, which is based on the conservative fragment of 16S rDNA, was consistent with the traditional morphology judgement, we confirmed that environmental factors have a huge impact on the distribution of Microcystis and the molecular biology techniques based on 16S rDNA sequencing were reliable for Microcystis identification.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
An J, Carmichael WW (1994) Use of a colorimetric protein phosphatase inhibition assay and enzyme linked immunosorbent assay for the study of microcystins and nodularins. Toxicon 32(12):1495–1507
Chen YQ, Zhuang JY, Zhuang L, Zeng LM, Qu LH (1999) Sequence analysis of rDNA 16-23S intergenic spacer from Microcystis aeruginosa ana Microcystis wesenbergii in Donghu Lake, China. Acta Hydrobiol Sin 23(1):41–45
Codd GA, Morrison LF, Metcalf JS (2005) Cyanobacterial toxins: risk management for health protection. Toxicol Appl Pharmacol 203(3):264–272
Davis TW, Berry DL, Boyer GL, Gobler CJ (2009) The effects of temperature and nutrients on the growth and dynamics of toxic and non-toxic strains of Microcystis during cyanobacteria blooms. Harmful Algae 8(5):715–725
Hawkins PR, Chandrasena NR, Jones GJ, Humpage AR, Falconer IR (1997) Isolation and toxicity of cylindrospermopsis raciborskii from an ornamental lake. Toxicon 35(3):341–346
Hu HI, Li YY, Wei YX, Zhu HZ, Chen JY, Shi ZX (1980) Freshwater algae in China. Science Press, Peking
Humbert JF, Duris-Latour D, Le Berre B, Giraudet H, Salençon MJ (2005) Genetic diversity in Microcystis populations of a French storage reservoir assessed by sequencing of the 16S-23S rRNA intergenic spacer. Microb Ecol 49(2):308–314
Jaiswal P, Singh PK, Prasanna R (2008) Cyanobacterial bioactive molecules—an overview of their toxic properties. Can J Microbiol 54(9):701–717
Li JR, Chen LD, Fu BJ, Zhang SR, Li GQ (2002) Temporal and spatial characteristics of non-point source N in surface water of yuqiao reservoir watershed. Sci Geogr Sin/Dili Kexue 22(2):242
Liu XB, Peng WQ, He GJ, Liu JL, Wang YC (2008) A coupled model of hydrodynamics and water quality for Yuqiao reservoir in Haihe river basin. J Hydrodyn Ser B 20(5):574–582
Neilan BA, Jacobs D, DelDot T, Blackall LL, Hawkins PR et al (1997) rRNA sequences and evolutionary relationships among toxic and nontoxic cyanobacteria of the genus Microcystis. Int J Syst Bacteriol 47:693–697. doi:10.1099/00207713-47-3-693
Nonneman D, Zimba PV (2002) A PCR-based test to assess the potential for microcystin occurrence in channel catfish production ponds 1, 2. J Phycol 38(1):230–233
Oh KH, Shin SH (2012) Simultaneous quantification of cyanobacteria and Microcystis spp. using real-time PCR. J Microbiol Biotechnol 22(2):248–255
Otsuka S, Suda S, Li R, Watanabe M, Oyaizu H, Matsumoto S, Watanabe MM (1999) Phylogenetic relationships between toxic and non-toxic strains of the genus Microcystis based on 16S to 23S internal transcribed spacer sequence. FEMS Microbiol Lett 172(1):15–21
Otten TG, Paerl HW (2011) Phylogenetic inference of colony isolates comprising seasonal Microcystis blooms in Lake Taihu, China. Microb Ecol 62(4):907–918
Peitong LFWHL (1988) The quantitative identification of nonpoint source pollution and its application in the yuqiao reservoir watershed. Acta Geogr Sin 4:004
Qian KC, Chen D, Lin SJ, Yuan ZQ, Hu R, Han BP (2005) The analysis of PC-IGS sequences from four freshwater Microcystis sp. Ecol Sci 24(2):150–153
Rouhiainen L, Vakkilainen T, Siemer BL, Buikema W, Haselkorn R, Sivonen K (2004) Genes coding for hepatotoxic heptapeptides (microcystins) in the cyanobacterium Anabaena strain 90. Appl Environ Microbiol 70(2):686–692
Tillett D, Parker DL, Neilan BA (2001) Detection of toxigenicity by a probe for the microcystin synthetase A gene (mcyA) of the cyanobacterial genus Microcystis: comparison of toxicities with 16S rRNA and phycocyanin operon (phycocyanin intergenic spacer) phylogenies. Appl Environ Microbiol 67(6):2810–2818
Wang J, Xie SL, Wang ZJ, Xu Y, Li RH (2011) Molecular diversity and microcystin production of Microcystis in Fenhe River of Taiyuan. J Lake Sci 23(4):505–512
Zhang SR, Chen LD, Fu BJ, Li J (2003) The risk assessment of nonpoint pollution of phosphorus from agricultural lands: a case study of Yuqiao reservoir watershed. Quat Sci 23(3):262–269
Zhang W, Lou I, Ung WK, Kong Y, Mok KM (2014) Application of PCR and real-time PCR for monitoring cyanobacteria, Microcystis spp. and Cylindrospermopsis raciborskii in Macau freshwater reservoir. Front Earth Sci 8(2):291–301
Zhao Y, Nan J, Cui FY, Guo L (2007) Water quality forecast through application of BP neural network at Yuqiao reservoir. J Zhejiang Univ Sci A 8(9):1482–1487
Acknowledgments
This work was supported by The International Science & Technology Cooperation Program of China (Grant No. 2013DFA71340) and Major National Science & Technology Project of Water Pollution Control and Management of China (Grant No. 012ZX07203-002).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Huo, D., Luo, Y., Nie, Y., Sun, J., Wang, Y., Qiao, Z. (2015). The Distribution Characteristics of Microcystis novacekii Based on 16S rDNA Sequence. In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_4
Download citation
DOI: https://doi.org/10.1007/978-3-662-46318-5_4
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-46317-8
Online ISBN: 978-3-662-46318-5
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)