Abstract
Corynebacterium glutamicum, a well-known producer in the amino acids industry, has become a potential platform organism for synthetic biology in industrial biotechnology. During l-citrulline biosynthesis, NADPH is required as a crucial cofactor. Production of l-citrulline requires 2 mol of NADPH per mole of l-citrulline. The strategy focused on engineering of the pentose phosphate pathway (PPP) flux by different genetic modifications. In this study, two NADPH-supplying strategies based on glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase was compared, and their influences on l-citrulline biosynthesis were examined. The zwf and gnd genes were overexpressed in the l-citrulline-producing strain CgΔargG. The expression of both genes greatly enhanced intracellular NADPH concentration and l-citrulline production. The concentration of intracellular NADPH was increased by 287 and 363 %, and the production of l-citrulline was increased by 30.8 and 20.5 % in CgΔargG/pXMJ19-zwf and CgΔargG/pXMJ19-gnd, respectively, compared with CgΔargG. The concentration of NADPH in a zwf- and gnd-expressing strain was increased by 287 and 363 %, respectively. These results are helpful for improving l-citrulline biosynthesis and other biosynthetic processes.
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Acknowledgments
This study was supported by the National Basic Research Program of China (973 Program, No. 2011CBA00807), National High Technology Research and Development Program of China (863 Project, No. 2012AA022101), National Natural Science Foundation of China (NSFC, No. 21406113), Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (No. 13KJB530008), and Scientific Research Innovation Projects of Jiangsu Province for University Graduate Students (No. KYLX_0777).
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Liu, Z. et al. (2015). Expression of Glucose-6-Phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase Improve l-Citrulline Biosynthesis in argG-Deleted Corynebacterium glutamicum . In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_22
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DOI: https://doi.org/10.1007/978-3-662-46318-5_22
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