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Cloning and Sequence Analysis of a Novel ACC Oxidase Gene from Peanut (Arachis hypogaea L.)

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Advances in Applied Biotechnology

Abstract

Ethylene plays important roles in seed dormancy. As a key enzyme in ethylene biosynthesis, ACC oxidase gene (ACCO) converts 1-aminocyclopropane-1-carboxylic acid (ACC) into ethylene. To study the mechanism underlying peanut seed dormancy, cDNA sequence of the candidate gene was isolated from peanut using RACE technique. As in the situation in Arabidopsis, the ACCO from peanut (AhACCO gene) contained four exons and three introns. Homology comparison analysis showed 68–87 % sequence similarity between AhACCO and ACCO genes of other selected plants.

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Acknowledgments

We would like to thank China Agricultural Research System (Grant No. CARS-14) and SAAS (Shandong Academy of Agricultural Sciences) Key Science & Technology Achievements Incubation Project (2014CPGY09) for providing financial support to this study.

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Correspondence to Chuantang Wang .

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Sun, Q. et al. (2015). Cloning and Sequence Analysis of a Novel ACC Oxidase Gene from Peanut (Arachis hypogaea L.). In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_10

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