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Prohibitin: Mitochondrial Tumor Suppressor Protein

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Mitochondrial DNA Mutations in Aging, Disease and Cancer

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Abstract

The prohibitin story begins with the development of an assay for the detection of mRNAs that could inhibit the initiation of DNA synthesis.1’2 Messenger RNAs were tested by microinjecting them into individual human diploid fibroblasts that had been growth-arrested by serum restriction. The cells were then stimulated to undergo DNA replication by addition of serum to the media. Cells that had entered S phase or replicated DNA would incorporate [3H] thymidine into their nuclei. The labeled cells could be visualized by autoradiography. Two sources of mRNA (senescent human diploid fibroblasts and normal rat liver) contained mRNAs that inhibited DNA synthesis. My work focused on the inhibitory mRNA that was detected in normal rat liver.

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Authors
  1. J. K. McClung
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Editors and Affiliations

  1. The Division of Experimental Therapeutics and Radiation Oncology, Johns Hopkins Oncology Center and Department of Environmental Health, Johns Hopkins School of Public Health, 600 N. Wolfe Street, Room 2-121, 21287, Baltimore, Maryland, USA

    Keshav K. Singh Ph.D.

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© 1998 Springer-Verlag Berlin Heidelberg

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McClung, J.K. (1998). Prohibitin: Mitochondrial Tumor Suppressor Protein. In: Singh, K.K. (eds) Mitochondrial DNA Mutations in Aging, Disease and Cancer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-12509-0_17

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  • DOI: https://doi.org/10.1007/978-3-662-12509-0_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-12511-3

  • Online ISBN: 978-3-662-12509-0

  • eBook Packages: Springer Book Archive

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