Zusammenfassung
Die Struktur und Sequenz des humanen Faktör-VIII-Gens (FVIII-Gens) wurde 1984 gleichzeitig von 2 verschiedenen Forschergruppen publiziert (Gitschier et al.; Toole et al). Beide Arbeitsgruppen benutzten als Ausgangsmaterial eine genomische cDNA-Bibliothek, die aus einer Zellinie mit tetraploidem Chromosomensatz — also einer Zelllinie mit 4 X-Chromosomen — gewonnen wurde. Aus dieser Bibliothek konnte mit Hilfe von Oligonukleotiden, die entsprechend der Sequenz bekannter FVIII-Peptidfragmente synthetisiert worden waren, ein FVIII-Gen in einer Größe von über 200 Kilobasen (kB) isoliert werden. Damit umfaßt das FVIII-Gen etwa 0,1% des gesamten X-Chromosoms.
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Tuddenham, E.G., Cooper, D.N. (1999). Faktor VIII: Molekularbiologie. In: Müller-Berghaus, G., Pötzsch, B. (eds) Hämostaseologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-07673-6_20
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