Skip to main content

Cryopreservation of Neem (Azadirachta indica A. Juss.) Seeds

  • Chapter
Cryopreservation of Plant Germplasm II

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 50))

Abstract

Neem, Azadirachta indica (A. Juss.), is a member of the Meliaceae which is believed to have emerged from the Indian sub-continent and Myanmar (Kundu and Tigersted 1997). These authors describe the introduction of this evergreen tree to different parts of the world, mainly in the drier tropical and sub-tropical zones of Asia, Africa, the Americas, Australia and the South Pacific Islands. Nowadays, neem introductions are so old and widely spread that knowledge of its original distribution has become uncertain (Farooqui et al. 1998).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bellefontaine R, Audinet M (1993) Les problèmes de semences forestières notamment en Afrique. In: Some L, de Kam M (eds) Tree seed problems, with special reference to Africa. Backhuis, Leiden, pp 268–274

    Google Scholar 

  • Berjak P, Campbell GK, Farrant JM, Omondi-Oloo W, Pammenter NW (1995) Responses of seeds of Azadirachta indica (neem) to short-term storage under ambient or chilled conditions. Seeds Sci Technol 23: 779–792

    Google Scholar 

  • Berjak P, Kioko JI, Norris M, Mycock DJ, Wesley-Smith J, Pammenter NW (1999a) Cryopreservation — an elusive goal? In: Marzalina M, Khoo KC, Jayanthi N, Tsan FY, Krishnapillay B (eds) Recalcitrant seeds. Proceeding of the IUFRO Seed Symposium, Forest Research Institute, Kuala Lumpur, Malaysia, pp 96–109

    Google Scholar 

  • Berjak P, Walker M, Watt MP, Mycock DJ (1999b) Experimental parameters underlying failure or success in plant germplasm cryopreservation: a case study on zygotic axes of Quercus robur L. Cryo Lett 20: 251–262

    Google Scholar 

  • Chaudhury R, Chandel KPS (1991) Cryopreservation of desiccated seeds of neem (Azadirachta indica A. Juss) for germplasm conservation. Ind J Plant Genet Resour 4: 67–72

    Google Scholar 

  • Chin HF (1994) Seedbanks: conserving the past for the future. Seed Sci Technol 22: 385–400

    Google Scholar 

  • Eeswara JP, Stuchbury T, Allan EJ, Mordue AJ (1998) A standard procedure for the micropropagation of the neem tree (Azadirachta indica A. Juss ). Plant Cell Rep 17: 215–219

    Article  CAS  Google Scholar 

  • Ermel K, Pahlich E, Schmutterer H (1987) Azadirachtin content of neem kernels from different geographical locations and its dependance on temperature, relative humidity and light. In: Schmutterer H,Ascher KRS (eds) Natural pesticides from the neem tree (Azadirachta indica A. Juss) and other tropical plants. Proceedings of the Third International Neem Conference, Nairobi. German Agency for Technical Cooperation, Eschborn, pp 171–184

    Google Scholar 

  • Farooqui N, Ranade S, Sane PV (1998) RAPD profile variation amongst provenances of neem. Biochem Mol Biol Int 45: 931–939

    PubMed  CAS  Google Scholar 

  • Gamene CS, Kraak HL, Van Pijlen JG, de Vos CHR (1996) Storage behaviour of neem (Azadirachta indica) seeds from Burkina Faso. Seed Sci Technol 24: 441–448

    Google Scholar 

  • Hong TD, Ellis RH (1996) A protocol to determine seed storage behaviour. IPGRI, Rome

    Google Scholar 

  • International Board for Plant Genetic Resources (1976) In: Report of IBPGR Working Group on engineering, design and cost aspects of long term storage facilities. Rome, Italy

    Google Scholar 

  • Kundu S, Tigersted PMA (1997) Geographical variation in seed and seedling traits of neem (Azadirachta indica A. Juss) amongst ten populations studied in growth chamber. Silvae Genet 46: 129–137

    Google Scholar 

  • Ley SV, Denholm AA, Wood A (1993) The chemistry of azadirachtin. Nat Product Rep 10: 109–157

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Murthy BNS, Saxena PK (1998) Somatic embryogenesis and plant regeneration of neem (Azadirachta indica A. Juss). Plant Cell Rep 17: 469–475

    Article  Google Scholar 

  • Mycock DJ, Berjak P (1990) Fungal contaminants associated with several homoiohydrous (recalcitrant) seed species. Phytophylactica 22: 413–418

    Google Scholar 

  • National Research Council (1992) Neem: a tree for solving global problems. National Academy Press, Washington, DC

    Google Scholar 

  • Pammenter NW, Greggains V, Kioko JI, Wesley-Smith J, Berjak P, Finch-Savage WE (1998) Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis. Seed Sci Res 8: 463–472

    Article  Google Scholar 

  • Roberts EH (1973) Predicting the storage life of seeds. Seed Sci Technol 1: 499–541

    Google Scholar 

  • Sacandé M, Hoekstra FA, van Pijlen JG (1998) A multifactorial study of conditions influencing longevity of neem (Azadirachta indica) seeds. Seed Sci Res 8: 473–482

    Article  Google Scholar 

  • Sacandé M, Hoekstra FA, van Aelst AC, De Vos CHR (2000) Is oxidative stress involved in the loss of neem seed viability? Seed Sci Res 10: 381–392

    Article  Google Scholar 

  • Shankara Bhat S, Sateesh MK, Devaki NS (1998) A new destructive disease of Neem (Azadirachta indica) incited by Phomopsis azadirachtae. Curr Sci 74: 17–19

    Google Scholar 

  • Shrikhande M, Thengane SR, Mascarenhas AF (1993) Somatic embryogenesis and plant regeneration in Azadirachta indica A. Juss. In Vitro Cell Dev Biol Plant 29: 38–42

    Article  Google Scholar 

  • Singh RP (1987) Comparison of antifeedant efficacy and extract yields from different parts and ecotypes of neem (Azadirachta indica A. Juss) tree. In: Schmutterer H, Ascher KRS (eds) Natural pesticides from the neem tree (Azadirachta indica A. Juss) and other tropical plants. Proceeding of the 3rd International Neem Conference, Nairobi. German Agency for Technical Cooperation, Eschborn, pp 171–184

    Google Scholar 

  • Su WW, Hwang WI, Kirn SY, Sagawa Y (1997) Induction of somatic embryogenesis in Azadirachta indica. Plant Cell Tissue Organ Culture 50: 91–95

    Article  Google Scholar 

  • Walters C, Engels J (1998) The effect of storing seeds under extremely dry conditions. Seed Sci Res 8: 3–8

    Article  Google Scholar 

  • Wesley-Smith J, Vertucci CW, Berjak P, Pammenter NW, Crane J (1992) Cryopreservation of desiccation-sensitive axes of Camellia sinensis in relation to dehydration, freezing rate and the thermal properties of tissue water. J Plant Physiol 140: 596–604

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Dumet, D., Berjak, P. (2002). Cryopreservation of Neem (Azadirachta indica A. Juss.) Seeds. In: Towill, L.E., Bajaj, Y.P.S. (eds) Cryopreservation of Plant Germplasm II. Biotechnology in Agriculture and Forestry, vol 50. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04674-6_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-04674-6_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-07502-5

  • Online ISBN: 978-3-662-04674-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics