Cryopreservation of Plant Germplasm II

1. Front Matter

   Pages I-XVI

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2. Cryopreservation of Plant Germplasm

   1. Front Matter

      Pages 1-1

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   2. Cryopreservation of Plant Germplasm: Introduction and Some Observations

      • Leigh E. Towill

      Pages 3-21

   3. Implementing Cryopreservation for Long-Term Germplasm Preservation in Vegetatively Propagated Species

      • Barbara M. Reed

      Pages 22-33

3. Herbaceous Species

   1. Front Matter

      Pages 35-35

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   2. Cryopreservation of Allium sativum L. (Garlic)

      • E. R. J. Keller

      Pages 37-47

   3. Cryopreservation of Apium graveolens L. (Celery) Seeds

      • M. Elena González-Benito, José María Iriondo

      Pages 48-56

   4. Cryopreservation of Armoracia rusticana P. Gaert., B. Mey. et Scherb. (Horseradish) Hairy Root Cultures

      • Kazumasa Hirata, Phunchindawan Monthana, Akira Sakai, Kazuhisa Miyamoto

      Pages 57-65

   5. Cryopreservation of Chamomilla recutita L. Rauschert (Chamomile) Callus

      • Eva Čellárová, Katarína Kimáková, Martina Urbanová

      Pages 66-77

   6. Cryopreservation of Cichorium intybus L. var. foliosum (Chicory)

      • B. Vandenbussche, M. Demeulemeester, M. De Proft

      Pages 78-95

   7. Cryopreservation of Colocasia esculenta L. Schott (Taro)

      • Nguyen Tien Thinh, Hiroko Takagi

      Pages 96-118

   8. Cryopreservation of Hordeum (Barley)

      • Jun-Hui Wang, Chun-Nong Huang

      Pages 119-135

   9. Cryopreservation of Humulus lupulus L. (Hop)

      • M. A. Revilla, D. Martínez

      Pages 136-150

   10. Cryopreservation of Mentha (Mint)

       • Leigh E. Towill

       Pages 151-163

   11. Cryopreservation of Panax (Ginseng)

       • Kayo Yoshimatsu, Koichiro Shimomura

       Pages 164-179

   12. Cryopreservation of In Vitro Grown Apical Shoot Tips of Wasabia japonica (Wasabi) by Different Procedures

       • Toshikazu Matsumoto

       Pages 180-195

4. Woody Species

   1. Front Matter

      Pages 197-197

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   2. Cryopreservation of Somatic Embryos from Aesculus hippocastanum L. (Horse Chestnut)

      • Z. Jekkel, J. Kiss, G. Gyulai, E. Kiss, L. E. Heszky

      Pages 199-212

   3. Cryopreservation of Neem (Azadirachta indica A. Juss.) Seeds

      • Dominique Dumet, Patricia Berjak

      Pages 213-219

   4. Cryopreservation of Coffea (Coffee)

      • S. Dussert, N. Chabrillange, E. Engelmann, F. Anthony, N. Vasquez, S. Hamon

      Pages 220-233

Ex situ preservation of germplasm for higher plant species has been accom­ plished using either seeds or clones, but storage of these under typical condi­ tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur­ vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com­ monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not haveoccurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry­ opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.

• Cryopreservation
• Embryo
• Gefrierkonservierung
• Gewebekultur
• Keimplasma
• Neem
• Pflanzen
• germplasm
• olive
• plants
• rose
• tissue culture
• woody species

• USDA-ARS National Center for Genetic Resources Preservation, Fort Collins, USA

  Leigh E. Towill

• New Delhi, India

  Y. P. S. Bajaj

Policies and ethics