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The Opsonic Capacity of Monoclonal Antibodies Against Escherichia Coli 0111 and Its Rough Mutant E. Coli J5

Complement activating and opsonic capacity of MAb

  • Conference paper
Bacteria, Complement and the Phagocytic Cell

Part of the book series: NATO ASI Series ((ASIH,volume 24))

  • 145 Accesses

Abstract

Six monoclonal antibodies raised against Escherichia coli 0111 and against its rough mutant (chemotype Rc) were studied. One IgG2A against E. coli J5, one IgM anti-J5 and one IgG2a anti-0111 monoclonal antibody did not bind to lipopolysaccharides of the homologous strain, but cross-reacted with heterologous gram-negative rods in an enzyme-linked immunosorbent assay. These three monoclonal antibodies activated complement when incubated with homologous or heterologous strains, but were opsonic neither in the presence nor in the absence of complement. The other three monoclonal antibodies were directed against lipopolysaccharide of the homologous strain, but showed no cross-reactivity. However, these antibodies cross-reacted when bacteria were exposed to low concentrations of antibiotics (monobactams). The IgG3 and one IgM anti-J5 monoclonal antibodies activated complement and were opsonic only in the presence of complement. The IgM anti-0111 monoclonal antibody activated complement and was opsonic both in the presence and absence of complement.

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Authors and Affiliations

  1. Laboratory of Microbiology, State University of Utrecht, Catharijnesingel 59, 3511 GG, Utrecht, The Netherlands

    J. Verhoef, R. W. Vreede & A. S. Bouter

Authors
  1. J. Verhoef
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  2. R. W. Vreede
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  3. A. S. Bouter
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Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, New York Medical College, 10595, Valhalla, NY, USA

    Felipe C. Cabello

  2. Institute of Microbiology, University of Genova, Viale Benedetto XV 10, 16132, Genova, Italy

    Carla Pruzzo

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© 1988 Springer-Verlag Berlin Heidelberg

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Verhoef, J., Vreede, R.W., Bouter, A.S. (1988). The Opsonic Capacity of Monoclonal Antibodies Against Escherichia Coli 0111 and Its Rough Mutant E. Coli J5. In: Cabello, F.C., Pruzzo, C. (eds) Bacteria, Complement and the Phagocytic Cell. NATO ASI Series, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-85718-8_22

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  • DOI: https://doi.org/10.1007/978-3-642-85718-8_22

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-85720-1

  • Online ISBN: 978-3-642-85718-8

  • eBook Packages: Springer Book Archive

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