The Opsonic Capacity of Monoclonal Antibodies Against Escherichia Coli 0111 and Its Rough Mutant E. Coli J5

Complement activating and opsonic capacity of MAb
  • J. Verhoef
  • R. W. Vreede
  • A. S. Bouter
Conference paper
Part of the NATO ASI Series book series (volume 24)

Abstract

Six monoclonal antibodies raised against Escherichia coli 0111 and against its rough mutant (chemotype Rc) were studied. One IgG2A against E. coli J5, one IgM anti-J5 and one IgG2a anti-0111 monoclonal antibody did not bind to lipopolysaccharides of the homologous strain, but cross-reacted with heterologous gram-negative rods in an enzyme-linked immunosorbent assay. These three monoclonal antibodies activated complement when incubated with homologous or heterologous strains, but were opsonic neither in the presence nor in the absence of complement. The other three monoclonal antibodies were directed against lipopolysaccharide of the homologous strain, but showed no cross-reactivity. However, these antibodies cross-reacted when bacteria were exposed to low concentrations of antibiotics (monobactams). The IgG3 and one IgM anti-J5 monoclonal antibodies activated complement and were opsonic only in the presence of complement. The IgM anti-0111 monoclonal antibody activated complement and was opsonic both in the presence and absence of complement.

Keywords

Bacillus Myeloma Gelatin Dextran Galactose 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • J. Verhoef
    • 1
  • R. W. Vreede
    • 1
  • A. S. Bouter
    • 1
  1. 1.Laboratory of MicrobiologyState University of UtrechtUtrechtThe Netherlands

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