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Interaction of Pseudomonas Aeruginosa with Complement

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Bacteria, Complement and the Phagocytic Cell

Part of the book series: NATO ASI Series ((ASIH,volume 24))

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Abstract

Pseudomonas aeruginosa is a ubiquitous gram-negative organism which is not ordinarily pathogenic for individuals with normal host defenses. However, this organism is a major cause of morbidity and mortality for patients with serious burn or wound injuries, neoplastic disease, granulocytopenia, immunological deficiencies or cystic fibrosis (CF), and for patients receiving intensive immunosuppressive chemotherapy (Bodey et al. 1983). Once established, this opportunistic pathogen can produce a variety of extracellular virulence factors, including toxins, hemolysins, aggressins, adherence factors, and a slime exopolysaccharide (prevalent amongst isolates from CF patients), any of which can contribute to the disease manifestations observed and/or to the resistance of this organism to host clearance mechanisms (Lory and Tai, 1985). Furthermore, infections with P. aeruginosa remain extremely difficult to control and/or eradicate with currently available antibiotics. As a result, a more comprehensive understanding of the interaction between this organism and host defense mechanisms is needed to guide efforts aimed at developing alternative prophylactic or immunotherapeutic treatment regimens.

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References

  • Baltimore, R.S. and Shedd, D.G. The role of complement in the opsonization of mucoid and non-mucoid strains of Pseudomnas aeruginosa. Pediat. Res. 17: 952–958 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Bjornson, A.B. and Michael, J.G. Factors in normal human serum that promote bacterial phagocytosis. J Infect Dis 130: S119–131 (1973).

    Article  Google Scholar 

  • Bodey, G.P., Bolivar, R., Fainstein, V. and Jadeja, L. Infections caused by Pseudomonas aeruginosa. Rev Infect Dis 5: 279–313 (1983).

    Article  PubMed  CAS  Google Scholar 

  • Borowski, R.S., Schiller, N.L. Examination of the bactericidal and opsonic activity of normal human serum for a mucoid and non-mucoid strain of Pseudomonas aeruginosa. Curr Microbiol 9: 25–30 (1983).

    Article  Google Scholar 

  • Engels, W., Endert, J., Kamps, M.A.F., and van Boven, C.P.A. Role of lipopolysaccharide in opsonization and phagocytosis of Pseudomonas aeruginosa. Infect Immun 49: 182–189 (1985a).

    PubMed  CAS  Google Scholar 

  • Engels, W., Endert, J., and van Boven, C.P.A. A quantitative method for assessing the third complement factor (C3) attached to the surface of opsonized Pseudomonas aeruginosa: interrelationship between C3 fixation, phagocytosis, and complement consumption. J Immunol Methods 81: 43–53 (1985b).

    Article  PubMed  CAS  Google Scholar 

  • Goldman, R.C., Joiner, K., and Leive, L. Serum-resistance mutants of Escherichia coli contain increased lipopolysaccharide, lack an 0 antigen capsule, and cover more of their lipid A core with 0 antigen. J Bacteriol 159: 877–882 (1984).

    PubMed  CAS  Google Scholar 

  • Gross, G.N., Rehm, S.R., and Pierce, A.K. The effect of complement deple- tion on lung clearance of bacteria. J Clin Invest 62: 373–378 (1978).

    Article  PubMed  CAS  Google Scholar 

  • Hammer, M.C., Baltch, A.L., Sutphen, N.T., Smith, R.P., and Conroy, J.V. Pseudomonas aeruginoa: quantitation of maximum phagocytic and bactericidal capabilities of normal human granulocytes. J Lab Clin Med 98: 938–948 (1981).

    PubMed  CAS  Google Scholar 

  • Hancock, R.E.W., Mutharia, L.M., Chan, L., Darveau, R.P., Speert, D.P., and Pier, G.B. Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide 0 side chains. Infect Immun 42: 170–177 (1983).

    PubMed  CAS  Google Scholar 

  • Heidbrink, P.J., Toews, G.B., Gross, G.N., and Pierce, A.K. Mechanisms of complement-mediated clearance of bacteria from the murine lung. Am Rev Respir Dis 125: 517–520 (1982).

    PubMed  CAS  Google Scholar 

  • Hoiby, N., and 011ing, S. Pseudomonas aeruginosa infection in cystic fibrosis. Bactericidal effect of serum from normal individuals and patients with cystic fibrosis on Pseudomonas aeruginosa strains from patients with cystic fibrosis and other diseases. Acta Path Micrbiol Scant Sect C 85: 107–114 (1977).

    Google Scholar 

  • Joiner, K.A., Hammer, C.H., Brown, E.J., Cole, R.J., and Frank, M.M. Studies on the mechanism of bacterial resistance to complement-mediated killing. I. Terminal complement components are deposited and released form Salmonella minnesota S218 without causing bacterial death. J Exp Med 155: 797–808 (1982a).

    Article  PubMed  CAS  Google Scholar 

  • Joiner, K.A., Hammer, C.H., Brown, E.J., and Frank, M.A. Studies on the mechanism of bacterial resistance to complement-mediated killing. II. C8 and C9 release C5b67 from the surface of Salmonella minnesota S218 because the terminal complex does not insert into bacterial outer membrane. J Exp Med 155: 809–815 (1982b).

    Article  PubMed  CAS  Google Scholar 

  • Joiner, K.A., Schmetz, M.A., Goldman, R.C., Leive, L., and Frank, M.M. Mechanism of bacterial resistance to complement-mediated killing: inserted C5b-9 correlates with killing for Escherichia coli 0111B4 varying in 0-antigen capsules and 0-polysaccharide coverage of lipid A core oligosaccharide. Infect Immun 45: 113–117 (1984).

    PubMed  CAS  Google Scholar 

  • Joiner, K.A., Grossman, N., Schmetz, M., and Leive, L. C3 binds preferentially to long-chain lipopolysaccharide during alternativeathway activation by Salmonella montevideo. J Immunol 136: 710–715 (1986).

    PubMed  CAS  Google Scholar 

  • Larsen, G.L., Mitchell, B.C., Harper, T.B., and Henson, P.M. The pulmonary response of C5 sufficient and deficient mice to Pseudomonas aeruginosa. Am Rev Respir Dis 126: 306–311 (1982).

    PubMed  CAS  Google Scholar 

  • Leive, L. Release of lipopolysaccharide by EDTA treatment of Escherichia coli. Biochem Biophys Res Commun 21: 290–296 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Lory, S., and Tai, P.C. Biochemical and genetic aspects of Pseudomonas aeruginosa virulence. Curr Top Microbiol Immunol 118: 53–69 (1985).

    Article  PubMed  CAS  Google Scholar 

  • Meshulam, T., Verbrugh, H., and Verhoeff, J. Serum induced lysis of Pseudomonas aeruginosa. Eur J Clin Microbiol 1: 1–6 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Murphey, S.A., Root, R.K., and Schreiber, A.D. The role of antibody and complement in phagocytosis by rabbit alveolar macrophages. J Infect Dis 140: 896–903 (1979).

    Article  PubMed  CAS  Google Scholar 

  • Muschel, L.H., Ahl, L.A., and Fisher, M.W. Sensitivity of Pseudomonas aeruginosa to normal serum and polymyxin. J Bacteriol 98: 453–457 (1969).

    PubMed  CAS  Google Scholar 

  • Muschel, L.H., and Larsen, L.J. The sensitivity of smooth and rough gram-negative bacteria to the immune bactericidal reaction. Proc Soc Exp Biol Med 133: 345–348 (1970).

    PubMed  CAS  Google Scholar 

  • Nelson, B.W., and Roantree, R.J. Analyses of lipopolysaccharides extracted from penicillin-resistant, serum-sensitive Salmonella mutants. J Gen Microbiol 48: 179–188 (1967).

    PubMed  CAS  Google Scholar 

  • Nguyen, B-YT, Peterson, P.K., Verbrugh, H.A., Quie, P.G., and Hoidal, J.R. Differences in phagocytosis and killing by alveolar macrophages from humans, rabbits, rats and hamsters. Infect Immun 36: 504–509 (1982).

    PubMed  CAS  Google Scholar 

  • Offredo-Hemmer, C., Berche, P., and Veron, M. A complement-sensitive mutant of Pseudomonas aeruginosa. Ann Microbiol (Paris) 134A: 281–294 (1983).

    Google Scholar 

  • Peterson, P.K., Kim, Y., Schmeling, D., Lindemann, M., Verhoef, J., and Quie, P.G. Complement-mediated phagocytosis of Pseudomonas aeruginosa. J Lab Clin Med 92: 883–894 (1978).

    PubMed  CAS  Google Scholar 

  • Pier, G.B., and Ames, P. Mediation of the killin and of rough, mucoid isolates of Pseudomonas aeruginosa from patients with cystic fibrosis by the alternative pathway of complement. J Infect Dis 150: 223–228 (1984).

    Article  PubMed  CAS  Google Scholar 

  • Reyes,.P., El-Khatib, M.R., Brown, W.J., Smith, F., and Lerner, A.M. Synergy between carbenicillin and an aminoglycoside (gentamicin or tobramycin) against Pseudomonas aeruginosa isolates from patients with endocarditis and sensitivity of isolates to normal human serum. J Infect Dis 140: 192–202 (1979).

    Article  Google Scholar 

  • Rowley, D. Sensitivity of rough gram-negative gram-negative bacteria to the bactericidal action of serum. J Bacteriol 95: 1647–1650 (1968).

    PubMed  CAS  Google Scholar 

  • Schiller, N.L., and Hatch, R.A. The serum sensitivity, colonial morphology, serogroup specificity, and outer membrane protein profile of Pseudomonas aerginosa strains isoalted from several clinical sites. Diagn Microbiol Infect Dic 1: 145–157 (1983).

    Article  CAS  Google Scholar 

  • Schiller, N.L., Alazard, M.J., and Borowski, R.S. Serum sensitivity of a Pseudomonas aeruginosa mucoid strain. Infect Immun 45: 748–755 (1984).

    PubMed  CAS  Google Scholar 

  • Schiller, N.L., Hackley, D.R., and Morrison, A. Isolation and characterization of serum-resistant strains of Pseudomonas aeruginosa derived from serum-sensitive parenteral strains. Curr Microbiol 10: 185–190 (1984).

    Article  CAS  Google Scholar 

  • Schiller, N.L., Joiner, K.A. Interaction of complement with serum-sensitive and serum-resistant strains of Pseudomonas aeruginosa. Infect Immun 54: 689–694 (1986).

    PubMed  CAS  Google Scholar 

  • Schiller, D.R., and Miller, K.D. Elastase of Pseudomonas aeruginosa: inactivation of complement components and complement-dervied chemotactic factors. Infect Immun 10: 128–135 (1974).

    Google Scholar 

  • Taylor, P.W., and Kroll, H-P. Interaction of human complement proteins with serum-sensitive and serum-resistant strains of Escherichia coli. Mol Immunol 21: 609–620 (1984).

    Article  PubMed  CAS  Google Scholar 

  • Thomassen, M.J., and Demko, C.A. Serum bactericidal effect on Pseudomonas aeruginosa isolates from cystic fibrosis patients. Infect Immun 33: 512–518 (1981).

    PubMed  CAS  Google Scholar 

  • Young, L.S., and Armstrong, D. Human immunity to Pseudomonas aeruginosa: I. In vitro interaction of bacteria, polymorphonuclear leukocytes, and serum factors. J Infect Dis 126:257–275 (1972).

    Google Scholar 

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Authors and Affiliations

  1. Division of Biomedical Sciences, University of California, Riverside, Riverside, CA, 92521, USA

    Neal L. Schiller

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  1. Neal L. Schiller
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Editors and Affiliations

  1. Department of Microbiology and Immunology, New York Medical College, 10595, Valhalla, NY, USA

    Felipe C. Cabello

  2. Institute of Microbiology, University of Genova, Viale Benedetto XV 10, 16132, Genova, Italy

    Carla Pruzzo

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© 1988 Springer-Verlag Berlin Heidelberg

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Schiller, N.L. (1988). Interaction of Pseudomonas Aeruginosa with Complement. In: Cabello, F.C., Pruzzo, C. (eds) Bacteria, Complement and the Phagocytic Cell. NATO ASI Series, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-85718-8_14

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  • DOI: https://doi.org/10.1007/978-3-642-85718-8_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-85720-1

  • Online ISBN: 978-3-642-85718-8

  • eBook Packages: Springer Book Archive

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