Abstract
The gene 5 product of bacteriophage fd is a small DNA binding protein of 9,800 daltons containing 87 amino acids (Alberts et al. 1972; Oey and Knippers 1972). Its primary physiological role is the stabilization and protection of the single-strand DNA daughter virions from duplex formation following replication in the host (Salstrom and Pratt 1971). Because of the highly cooperative nature of its binding to DNA, it has the capacity to unwind or destabilize native DNA. Under low ionic strength conditions in vitro, it will melt double-stranded homopolymers and will reduce the melting temperature of native double-strand calf thymus DNA by 40°C (Salstrom and Pratt 1971).
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References
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McPherson, A., Wang, A., Jurnak, F., Molineux, I., Rich, A. (1980). Structure of the Gene 5 DNA Binding Protein from Bacteriophage fd and its DNA Binding Cleft. In: Chapeville, F., Haenni, AL. (eds) Chemical Recognition in Biology. Molecular Biology, Biochemistry and Biophysics, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-81503-4_19
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DOI: https://doi.org/10.1007/978-3-642-81503-4_19
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