Abstract
The processing of nuclear pre-mRNA is an important step in regulating the expression of genes transcribed by RNA polymerase II. These processing events include capping, splicing, 3’-end processing and transport from the nucleus to the cytoplasm. Transcripts of RNA polymerase II are assembled during transcription with proteins and other components into a ribonucleoprotein (RNP) complex and remain associated in RNP particles throughout their residence in the nucleus (Miller and Hamkalo 1972; Sommerville 1981; Wu et al. 1991). It can be logically assumed that the processing events occur within these nuclear RNP complexes. Therefore, the isolation and characterization of RNP particles that package intact nuclear RNA should shed light on the characteristics of the pre-mRNA processing machinery of the living cell.
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Sperling, R., Sperling, J. (1998). The InRNP Particle — A Naturally Assembled Complex of Pre-mRNA and Splicing Factors. In: Schenkel, J. (eds) RNP Particles, Splicing and Autoimmune Diseases. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80356-7_2
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DOI: https://doi.org/10.1007/978-3-642-80356-7_2
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