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  • © 1998

RNP Particles, Splicing and Autoimmune Diseases

  • A practical tool for anyone working at the lab bench
  • Easy to follow step-by-step protocols
  • Background information and full practical details

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (8 chapters)

  1. Front Matter

    Pages I-X
  2. Isolation and Immunochemical Characterization of hnRNP Particles

    • Frank Jung, Constantin E. Sekeris, Johannes Schenkel
    Pages 1-28
  3. Procedures for Three-Dimensional Reconstruction from Thin Sections with Electron Tomography

    • Ulf Skoglund, Lars-Göran Öfverstedt, Bertil Daneholt
    Pages 72-94
  4. Detection of Autoantibodies to Extractable Cellular Antigens

    • Panayiotis G. Vlachoyiannopoulos
    Pages 141-154
  5. Methods in Immunolocalization of Autoantigens

    • Martin Blüthner
    Pages 155-183
  6. In Vitro Splicing of Pre-mRNA in HeLa Extracts

    • Johannes Schenkel, Frank Jung, Apostolia Guialis, Angela Krämer
    Pages 184-210
  7. Back Matter

    Pages 211-225

About this book

The maturation of the precursors of mRNA to a functional transcript in eukaryotic cells is a complex, multistep process. It includes the formation of RNP complexes and the splicing procedure. Also, the presence of snRNA packaged as snRNP particles plays an important role in this process. In addition, snRNPs are involved in various autoimmune diseases in which autoantibodies are directed against components of these particles.
Relevant techniques to investigate RNA processing, splicing, protein-RNA complex formation and protein-RNA interactions as well as the use of autoantibodies in basic and clinical research are presented in this manual.

Editors and Affiliations

  • II. Physiologisches Institut, Universität Heidelberg, Heidelberg, Germany

    Johannes Schenkel

Bibliographic Information

  • Book Title: RNP Particles, Splicing and Autoimmune Diseases

  • Editors: Johannes Schenkel

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-80356-7

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1998

  • Softcover ISBN: 978-3-642-48975-4Published: 18 July 2012

  • eBook ISBN: 978-3-642-80356-7Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: X, 225

  • Number of Illustrations: 90 b/w illustrations

  • Topics: Cell Biology, Biochemistry, general, Molecular Medicine

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access