Abstract
Fullerenes are a new modification of pure carbon in addition to graphite and diamond. The first types to become known were called “Buckyballs”. They consist of cage-like molecules with a well defined number of carbon atoms (e.g. 60 atoms: C60). The Fullerenes are readily soluble in many organic solvents and vaporizable. A wide range of technological and also biomedical applications is expected for these molecules. First studies on in vitro, effects of C60 Fullerenes showed that the particles could induce the release of pro-inflammatory mediators by alveolar-macrophages (AM). However, this early material still might have contained about 10% of C70 and solvents due to the preparation procedure used at this time. Meanwhile, pure and solvent free C60 particles became available. One of the aims of the present study was to reevaluate our previous data with really pure material. In addition, the hemolytic properties were tested and the interaction of C60 with superoxide anion production was investigated.
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Literature
Brückner-Nieder U, Schmidt A, Schimmelpfeng J and Seidel A (1992) HL-60-derived Macrophages Are Resistant to Cytotoxic Effects of Quartz in; Environmental Hygiene I II ( Ed.: Seemayer, N.H, and Hadngy, W.), Springer, Berlin
Dubois CM Bissonette B and Rola-Pleszczynski M (1989) Asbestos Fibres and Silica Particles Stiumlate Rat Alveolar Macrophages to Release Tumor Necrosis Factor Am. Rev. Resp. Dis., 139: 1257–1264
Eskandari MK, Nguyen DT, Kunkel SL and Remick DG (1990) WEHI164 Subclone 13 Assay for TNF: Sensitivity, Specificity and Reliability Immuno. Invest., 19: 69–79
Espevik T and Nissen-Meyer M (1986) A Highly Sensitve Cell Line, WEHI 164 Clone 13 for Measuring Cytotoxic Factor/Tumor Necrosis Factor from Human Monocytes J. Immunol. Meth., 95: 99–105
Fisher GL, McNeill KL and Democko CJ (1983) Application of Bovine Alveolar Macrophage Bioassays in the Analysis of Toxic Agents in Complex Environmental Mixtures in: Short Term Bioassays in the Analysis of Complex Environmental Mixtures III (Ed.: Waters MD, Sandhu SS, Lewtas J, Claxton L, Chernoff N and Nesnow S ), Plenum Press, N.Y., 257–268
Fox ML (1973) The Bovine Alveolar Macrophage., I. Isolation, in vitro, Cultivation, Ultrastructure and Phagocytosis Can. J. Microbiol., 19: 1207–1210
Hanks JH and Wallace JH (1958) Determination of Cell Viability Proc. Soc. Exptl. Biol. Med., 98: 156–160
Johnston RB Jr. (1981) Secretion of Superoxide Anion in: Methods for Studying Mononuclear Phagocytes (Ed.: Adams PO, Edelson PJ and Karen H ), Academic Press, N.Y., 489–497
Krätschmer W, Lamb LD, Fostiropoulos K and Huffman DR (1990) Solid C60: A New Form of Carbon Nature, 318: 354–358
Nolan RP, Langer AM, Harington JS, Oster G and Selikoff IJ (1981) Quartz Hemolysis as Related to its Surface Functionalities Environmental Research, 26: 503–520
Rossi P, Chini L, Fattorossi A, Gidlund M, Galli E, Laan K, Jondal Mand Wigzell H (1987) 1,25-Dihydroxyvitamin D3 and Phorbolesters (TPA) May Induce Select in vivo, Differentiation Pathways in the HL-60 Promyelocyte Cell Line Clin. Immunol. Immunopathol., 44: 308–316
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© 1994 Springer-Verlag Berlin Heidelberg
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Baierl, T., Drosselmeyer, E., Seidel, A. (1994). Biological Effects of a New Type of Pure Carbon (Fullerenes) in vitro . In: Davis, J.M.G., Jaurand, MC. (eds) Cellular and Molecular Effects of Mineral and Synthetic Dusts and Fibres. NATO ASI Series, vol 85. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79041-6_14
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DOI: https://doi.org/10.1007/978-3-642-79041-6_14
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