Abstract
Achilles’ cleavage (AC) is a general method for combining the cleavage specificity of a restriction endonuclease with the binding specificity of other DNA-binding molecules. Restriction enzymes, which typically have recognition sequences of from 4 to 8 base pairs (bp) in length, cleave unmodified DNA from complex genomes at thousands or even millions of sites. By limiting the activity of these enzymes to a specific subset of these sites, the AC process makes it possible to cleave even the human genome at only a single site. The AC process can be used both as a means of obtaining basic information about the DNA-binding characteristics of the binding molecule employed and as a practical tool for creating unique cleavage specificities to analyze and manipulate DNA.
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© 1994 Springer-Verlag Berlin Heidelberg
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Koob, M., Szybalski, W. (1994). Achilles’ Cleavage: Conferring the Specificities of DNA-Binding Molecules on Restriction Endonucleases. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78666-2_16
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DOI: https://doi.org/10.1007/978-3-642-78666-2_16
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