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Resolvase-Mediated Site-Specific Recombination

  • N. D. F. Grindley
Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 8)

Abstract

Conservative site-specific recombination involves the reciprocal exchange of DNA segments by precise breakage and rejoining processes that involve no loss or synthesis of DNA. In principle, such events can occur intermolecularly (resulting in fusion of the two recombining partners) or intramolecularly (resulting in inversion or excision of one DNA segment relative to the other), although in most biological systems this directionality is strictly controlled. Biological roles of site-specific recombination include chromosomal integration and excision of phage genomes, monomerization of plasmid chromosomes, alternation of gene expression, resolution of transposition intermediates, and fusion of gene cassettes into a functional gene.

Keywords

Recombination Site Strand Exchange Synaptic Complex Crossover Site Half Twist 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • N. D. F. Grindley
    • 1
  1. 1.Department of Molecular Biophysics and BiochemistryYale UniversityNew HavenUSA

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