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Resolvase-Mediated Site-Specific Recombination

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Nucleic Acids and Molecular Biology

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 8))

Abstract

Conservative site-specific recombination involves the reciprocal exchange of DNA segments by precise breakage and rejoining processes that involve no loss or synthesis of DNA. In principle, such events can occur intermolecularly (resulting in fusion of the two recombining partners) or intramolecularly (resulting in inversion or excision of one DNA segment relative to the other), although in most biological systems this directionality is strictly controlled. Biological roles of site-specific recombination include chromosomal integration and excision of phage genomes, monomerization of plasmid chromosomes, alternation of gene expression, resolution of transposition intermediates, and fusion of gene cassettes into a functional gene.

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Authors and Affiliations

  1. Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06520-8114, USA

    N. D. F. Grindley

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  1. N. D. F. Grindley
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Editors and Affiliations

  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, Dundee, DD1 4HN, UK

    David M. J. Lilley

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© 1994 Springer-Verlag Berlin Heidelberg

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Grindley, N.D.F. (1994). Resolvase-Mediated Site-Specific Recombination. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78666-2_14

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  • DOI: https://doi.org/10.1007/978-3-642-78666-2_14

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