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Structure and Function of T4 Endonuclease V

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Nucleic Acids and Molecular Biology

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 8))

Abstract

Ultraviolet (UV) light causes photolesions in DNA that induce genetic mutations (Friedberg 1985; Hutchinson 1987; Sage 1993). One of the well-characterized photolesions is cyclobutane pyrimidine dimers, which are produced by irradiation at wavelengths close to the absorption maximum of DNA (about 260 nm). This photoproduct has a cyclobutane ring formed by covalent bonds at the C5 and C6 positions between two adjacent pyrimidine bases, and the major isomer in DNA duplexes in cis-syn (Scheme 1). This type of lesion has been reported to induce mutations in vivo (Jiang and Taylor 1993; Kamiya et al. 1993). UV irradiation predominantly induces transitions from cytosine to thymine at sequences of adjacent pyrimidines (Miller 1985; Schaaper et al. 1987; Jiang and Taylor 1993). In our study, however, transversions from thymine to adenine occurred most frequently on the 3′-side of the cis-syn thymine dimer or on the 5′-side of the trans-syn thymine dimer, and these mutations in the ras oncogene induced focus-formation of NIH3T3 cells (Kamiya et al. 1993).

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, 060, Japan

    S. Iwai & E. Ohtsuka

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  1. S. Iwai
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  2. E. Ohtsuka
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Editors and Affiliations

  1. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Straße 3, 37075, Göttingen, Germany

    Fritz Eckstein

  2. Biochemistry Department, University of Dundee, Dundee, DD1 4HN, UK

    David M. J. Lilley

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© 1994 Springer-Verlag Berlin Heidelberg

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Iwai, S., Ohtsuka, E. (1994). Structure and Function of T4 Endonuclease V. In: Eckstein, F., Lilley, D.M.J. (eds) Nucleic Acids and Molecular Biology. Nucleic Acids and Molecular Biology, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78666-2_12

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  • DOI: https://doi.org/10.1007/978-3-642-78666-2_12

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