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Stimulation of Phospholipase C by G-Protein βγ Subunits

  • P. Gierschik
  • M. Camps
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 108 / 2)

Abstract

The hydrolysis by phospholipase C of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) to inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol is a key mechanism by which many extracellular signaling molecules (e.g., hormones, growth factors, and neurotransmitters) regulate functions of their target cells (Berridge and Irvine 1989; Nishizuka 1988). There is ample evidence to suggest that many of the receptors interacting with these mediators stimulate phospholipase C via a guanine nucleotide binding protein (G-protein) (Cockcroft and Stutchfield 1988; De Vivo and Gershengorn 1990). In certain cell types, e.g., neutrophils and cultured granulocytes like HL-60 cells (Ohta et al. 1985; Krause et al. 1985), mast cells (Nakamura and Ui 1985), or renal mesangial cells (Pfeilschifter and Bauer 1986), the relevant G-protein appears to be a substrate for ADP-ribosylation by pertussis toxin. In other cells, e.g., cultured chick heart cells (Masters et al. 1985), 1321N1 astrocytoma cells (Hepler and Harden 1986), GH3 pituitary cells (Martin et al. 1986), or hepatocytes (Uhing et al. 1986) stimulation of phospholipase C is mediated by G-protein(s) resistant to modification by pertussis toxin. Recently, evidence has been presented suggesting that, in the latter systems, PLCβ1, but not PLCγ1 or PLGδ1, is activated by members of the newly discovered αq subfamily of the G-protein α subunits (see Chaps. 58 and 67 by Exton and Park, this volume, for review and references). With regard to stimulation of phospholipase C by pertussis-toxin-sensitive G-proteins, however, little information has been available until recently on the nature the G-protein involved.

Keywords

Adenylyl Cyclase Pertussis Toxin Formyl Peptide Receptor Renal Mesangial Cell Adenylyl Cyclase Inhibition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • P. Gierschik
  • M. Camps

There are no affiliations available

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