Monocyte-Conditioned Medium Factor as Auto-activation Signal for Monocyte Tumor Cytotoxicity

  • H. H. Spanjer
  • H. M. Dilling
  • T. G. J. de Boer
  • G. B. Humphrey
  • W. A. Kamps
Conference paper


The usefulness of tumoricidal macrophages as cancer treatment modality has been studied previously. Recently, cancer researchers have gained much interest in the use of biological response modifiers; therefore we, like others [1, 2], wanted to study the feasibility of using tumoricidal macrophages as an additional modality for the treatment of cancer. We studied the effects of two immunomodulators, lipopolysaccharide (LPS) and human recombinant interferon-γ (IFN-γ) on the development of tumor cytotoxicity in freshly isolated and cultured human monocytes. In our system, freshly isolated monocytes showed very low levels of tumor cytotoxicity when the effector cells were cultured in a medium supplemented with autologous serum. Enhanced tumor cytotoxicity was only observed when LPS was added to the culture medium; IFN-γ did not enhance tumor cytotoxicity at all. The addition of both LPS and IFN-γ did result in cytotoxicity levels equivalent to those obtained with LPS alone, indicating neither additive nor synergistic effects of the two immunomodulators.


Autologous Serum Biological Response Modifier Tumoricidal Activity Centrifugal Elutriation Separation Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Stevenson HC, Stevenson GW (1987) Adoptive cellular cancer immunotherapy. In:Oldham RK (ed) Principals of cancer biotherapy. Raven, New York, pp 385–397Google Scholar
  2. 2.
    Fidler IJ (1985) Macrophages and metastasis — a biological approach to cancer therapy:presidential address. Cancer Res 45:4714–4726PubMedGoogle Scholar
  3. 3.
    Plas A, de Witte T, Wessels H, Haanen C (1988) A new multichamber counterflow centrifuge rotor with high-separation capacity and versatile potentials. Exp Hematol 16:355–359Google Scholar
  4. 4.
    Figdor CG, Bont WS, Touw I, de Roos J, Roosnek EE, de Vries JE (1982) Isolation of functionally different human monocytes by counterflow centrifugal elutriation. Blood 60:46–53.PubMedGoogle Scholar
  5. 5.
    Adams DO, Edelson PJ, Koren H (eds) (1981) Methods for studying mononuclear phagocytes. Academic, New YorkGoogle Scholar
  6. 6.
    Nathan CF (1987) Secretory products of macrophages. J Clin Invest 79:319–326PubMedCrossRefGoogle Scholar
  7. 7.
    Old LJ (1988) Tumor necosis factor. Sci Am May:41–49Google Scholar

Copyright information

© Springer-Verlag, Berlin Heidelberg 1990

Authors and Affiliations

  • H. H. Spanjer
  • H. M. Dilling
  • T. G. J. de Boer
  • G. B. Humphrey
  • W. A. Kamps

There are no affiliations available

Personalised recommendations