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Monocyte-Conditioned Medium Factor as Auto-activation Signal for Monocyte Tumor Cytotoxicity

  • H. H. Spanjer
  • H. M. Dilling
  • T. G. J. de Boer
  • G. B. Humphrey
  • W. A. Kamps
Conference paper

Abstract

The usefulness of tumoricidal macrophages as cancer treatment modality has been studied previously. Recently, cancer researchers have gained much interest in the use of biological response modifiers; therefore we, like others [1, 2], wanted to study the feasibility of using tumoricidal macrophages as an additional modality for the treatment of cancer. We studied the effects of two immunomodulators, lipopolysaccharide (LPS) and human recombinant interferon-γ (IFN-γ) on the development of tumor cytotoxicity in freshly isolated and cultured human monocytes. In our system, freshly isolated monocytes showed very low levels of tumor cytotoxicity when the effector cells were cultured in a medium supplemented with autologous serum. Enhanced tumor cytotoxicity was only observed when LPS was added to the culture medium; IFN-γ did not enhance tumor cytotoxicity at all. The addition of both LPS and IFN-γ did result in cytotoxicity levels equivalent to those obtained with LPS alone, indicating neither additive nor synergistic effects of the two immunomodulators.

Keywords

Autologous Serum Biological Response Modifier Tumoricidal Activity Centrifugal Elutriation Separation Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag, Berlin Heidelberg 1990

Authors and Affiliations

  • H. H. Spanjer
  • H. M. Dilling
  • T. G. J. de Boer
  • G. B. Humphrey
  • W. A. Kamps

There are no affiliations available

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