Abstract
The bone marrow origin of natural killer (NK) cells has been well established, but the lineage of its progenitor is unknown. Mature NK cells express surface antigens which are also found on T lymphocytes, mature and immature myeloid cells and monocytes, suggesting that NK cell progenitors may share features common to lymphoid and myeloid precursors. Several growth and differentiation factors are known to be active on multipotential progenitors; these include IL-1, IL-3, and IL-4. Two of these cytokines, IL-1 and IL-4, have additionally been shown to affect mature NK cells, and in murine systems IL-3 inhibits a negative regulatory activity on NK cell progenitors [1–6]. IL-2 is the only factor known to be active in inducing the differentiation of NK cell precursors contained in human or murine marrow [7, 8]. We have investigated the effect of IL-2, alone and in combination with IL-1, IL-3, and IL-4 on the generation of NK cells from their earliest marrow precursors. To eliminate the effect of contaminating mature peripheral NK cells, we have tested marrow depleted of mature cells, including CD2+ NK and T cells, by treatment with soybean agglutinin and sheep erythrocytes.
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References
Ben Aribia MH, Leroy E, Lantz O, Metivier D, Autran B, Charpentier B, Hercend T, Senik A (1987) rIL 2-induced proliferation of human circulating NK cells and T lymphocytes:synergistic effects of IL 1 and IL 2. J Immunol 139:443–451
Crump WL, Owen-Schaub LB, Grimm EA (1989) Synergy of human recombinant interleukin 1 with interleukin 2 in the generation of lymphokine-activated killer-cells. Cancer Res 49:145–153
Spits H, Yssel H, Paliard X, Kastelein R, Figot C, De Vries JE (1988) IL-4 inhibits IL-2 mediated induction of human lymphokine-activated killer cells, but not the generation of antigen-specific cytotoxic T lymphocytes in mixed leukocyte cultures. J Immunol 141:29–36
Nagler A, Lanier LL, Phillips JH (1988) The effects of IL-4 on human natural-killer cells. A potent regulator of IL-2 activation and proliferation. J Immunol 141:2349–2351
Mule JJ, Krosnick JA, Rosenberg SA (1989) IL-4 regulation of murine lymphokine-activated killer activity in vitro. Effects on the IL-2 induced expansion, cytotoxicity, and phenotype of lymphokine-activated killer effectors. J Immunol 142:726–733
Kalland T (1987) Physiology of natural killer cells. In vivo regulation of progenitors by interleukin 3. J Immunol 139:3671–3675
Lotzova E, Savary CA (1987) Generation of NK cell activity from human bone marrow. J Immunol 139:279–284
Koo GC, Peppard JR, Mark WH (1984) Natural killer cells generated from bone marrow culture. J Immunol 132:2300–2304
Reisner Y, Kapoor N, Kirkpatrick D, Pollack MS, Dupont B, Good RA, O’Reilly RJ (1981) Transplantation for acute leukaemia with HLA-A and B non-identical parental marrow cells fractionated with soybean agglutinin and sheep red blood cells. Lancet II:327–331
Keever CA, Welte K, Small T, Levick J, Sullivan M, Hauch M, Evans RL, O’Reilly RJ (1987) Interleukin 2-activated killer cells in patients following transplants of soybean lectin-separated and E rosette-depleted bone marrow. Blood 70:1893–1903
Pross HF, Maroun J A (1984) The standardization of NK cell assays for use in studies of biological response modifiers. J Immunol Methods 68:235–249
Migliorati G, Cannarile L, Herberman RB, Bartocci A, Stanley ER, Riccardi C (1987) Role of interleukin 2 (IL 2) and hemopoietin-1 (H-l) in the generation of mouse natural killer (NK) cells from primitive bone marrow precursors. J Immunol 138:3618–3625
Siegel JP, Sharon M, Smith PL, Leonard WJ (1987) The IL-2 receptor b chain (p70):Role in mediating signals for LAK, NK, and proliferative activities. Science 238:75–78
Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH (1986) The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol 136:4480–4486
Spits H, Yssel H, Takebe Y, Arai N, Yokota T, Lee F, Arai K, Banchereau J, de Vries JE (1987) Recombinant interleukin 4 promotes the growth of human T cells. J Immunol 139:1142–1147
Han X, Itoh K, Balch CM, Pellis NR (1988) Recombinant interleukin 4 (RIL4) inhibits interleukin 2-induced activation of peripheral blood lymphocytes. Lymphokine Res 7:227–235
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© 1990 Springer-Verlag, Berlin Heidelberg
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Keever, C.A., Gazzola, M.V., Pekle, K., Bourhis, J.H., Gillio, A. (1990). Regulatory Effect of Recombinant Cytokines on NK Cell Differentiation from Early Marrow Precursors. In: Freund, M., Link, H., Welte, K. (eds) Cytokines in Hemopoiesis, Oncology, and AIDS. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75510-1_32
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DOI: https://doi.org/10.1007/978-3-642-75510-1_32
Publisher Name: Springer, Berlin, Heidelberg
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