Regulatory Effect of Recombinant Cytokines on NK Cell Differentiation from Early Marrow Precursors

  • C. A. Keever
  • M. V. Gazzola
  • K. Pekle
  • J. H. Bourhis
  • A. Gillio
Conference paper

Abstract

The bone marrow origin of natural killer (NK) cells has been well established, but the lineage of its progenitor is unknown. Mature NK cells express surface antigens which are also found on T lymphocytes, mature and immature myeloid cells and monocytes, suggesting that NK cell progenitors may share features common to lymphoid and myeloid precursors. Several growth and differentiation factors are known to be active on multipotential progenitors; these include IL-1, IL-3, and IL-4. Two of these cytokines, IL-1 and IL-4, have additionally been shown to affect mature NK cells, and in murine systems IL-3 inhibits a negative regulatory activity on NK cell progenitors [1–6]. IL-2 is the only factor known to be active in inducing the differentiation of NK cell precursors contained in human or murine marrow [7, 8]. We have investigated the effect of IL-2, alone and in combination with IL-1, IL-3, and IL-4 on the generation of NK cells from their earliest marrow precursors. To eliminate the effect of contaminating mature peripheral NK cells, we have tested marrow depleted of mature cells, including CD2+ NK and T cells, by treatment with soybean agglutinin and sheep erythrocytes.

Keywords

Fractionation HEPES Fluorescein FITC Progeni 

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Copyright information

© Springer-Verlag, Berlin Heidelberg 1990

Authors and Affiliations

  • C. A. Keever
  • M. V. Gazzola
  • K. Pekle
  • J. H. Bourhis
  • A. Gillio

There are no affiliations available

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