Abstract
Granulocyte-macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), and interleukin-3 (IL-3) are glycoproteins of known composition involved at different levels in the proliferation and maturation of human hematopoietic precursor cells [1, 2]. The complementary DNAs to their respective mRNAs have been cloned, and recombinant GM-CSF, G-CSF, and IL-3 are currently used in early clinical trials to alleviate chemotherapy-induced bone marrow toxicity [1]. However, recent evidence indicates that these cytokines might also stimulate in vitro growth of human solid tumor cell lines [3, 4]. Also, a variety of human solid tumors has been reported to produce CSFs which might in turn stimulate their own growth by paracrine or autocrine mechanisms [5–8, 23]. The purpose of the present study was to evaluate the effect of GM-CSF, G-CSF, and IL-3 on clonogenic cells from freshly obtained human solid tumor specimens using a capillary soft-agar cloning system.
This work was supported in part by a grant from the Behring Werke AG, Marburg, FRG, and by Deutsche Forschungsgemeinschaft grant Ha 1347/2-1.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Clark SE, Kamen R (1987) The human hematopoietic colony-stimulating factors. Science 236:1229
Dinarello CA, Mier JW (1987) Lymphokines. N Engl J Med 317:940
Berdel WE, Dannhauser-Riedl S, Steinhauser G, Winton EF:Various human hematopoietic growth factors (Interleukin-3, GM-CSF, G-CSF) stimulate clonal growth of nonhematopoietic tumor cells. Blood 73:80
Baldwin GC, DiPersio J, Kaufman SE, Quan SG, Golde DW, Gasson JC (1987) Characterization of human GM-CSF receptors on non-hematopoietic cells. Blood [suppl] 70:166
Takahashi M, Fujiwara M, Kishi K, Sakai C, Snada M, Moriyama Y, Shibata (1985) CSF producing gallbladder cancer:case report and characteristics of the CSF produced by tumor cells. Int J Cell Cloning 3:294
Wu MC, Cini JK, Yunis A A (1979) Purification of a colony-stimulating factor from cultured pancreatic carcinoma cells. J Biol Chem 254:6226
Okabe T, Fujisawa M, Kudo H, Honma H, Ohsawa N, Takaku F (1984) Establishment of a human colony-stimulating-factor-producing cell line from an undifferentiated large cell carcinoma of the lung. Cancer 54:1024
Takeda A, Suzumori K, Sugimoto Y, Yagami Y, Miyazawa T, Yamada C, Matsuyama M (1984) Clear cell carcinoma of the ovary with colony-stimulating-factor production. Cancer 54:1019
Hanauske A-R, Hanauske U, Von Hoff DD (1985) The human tumor cloning assay in cancer research and therapy. Curr Probl Cancer 9:1
Hanauske U, Hanauske A-R, Marshall MH, Muggia VA, Von Hoff DD (1987) Biphasic effect of vanadium salts on in vitro tumor colony growth. Int J Cell Cloning 5:170
Clark GM, Von Hoff DD (1983) Statistical considerations for in vitro/in vivo correlations using a cloning system In:Dendy P, Hill BT (eds) Human tumor drug sensitivity testing in vitro. Academic, New York, p 225
Antoniades HN, Owen A J (1982) Growth factors and regulation of cell growth. Ann Rev Med 33:445
Roberts AB, Frolik ChA, Anzano MA, Sporn MB (1983) Transforming growth factors from neoplastic and nonneoplastic tissues. Fed Proc 42:2621
Sporn MB, Roberts AB (1985) Autocrine growth factors and cancer. Nature 313:745
Lawrence DA (1985) Transforming growth factors — an overview. Biol. Cell 53:93
Goustin AS, Leof EB, Shipley GD, Moses HL (1986) Growth factors and cancer. Cancer Res 46:1015
Sporn MB, Todaro GJ (1980) Autocrine secretion and malignant transformation of cells. N Engl J Med 303:878
Pathak MA, Matrisian LM, Magun Be, Salmon SE (1982) Effect of epidermal growth factor on clonogenic growth of primary human tumor cells. Int J Cancer 30:745
Hamburger AW, White CP, Dunn FE, Citron ML, Hummel S (1983) Modulation of human tumor colony growth in soft agar by serum. Int J Cell Cloning 1:216
Hamburger AW, White CP, Brown RW (1981) Effect of epidermal growth factor on proliferation of human tumor cells in soft agar. J Natl Cancer Inst 67:825
Singletary SE, Tomasovic B, Spitzer G, Tucker SL, Hug V, Drewinko B (1985) Effects of epidermal growth factor, insulin, hydrocortisone, and estradiol on the cloning of human tumor cells. Int J Cell Cloning 3:407
Hanauske A-R, Hanauske U, Buchok J, Von Hoff DD (1988) Recombinant human transforming growth factor alpha stimulates in vitro colony formation of fresh human tumor specimens. Int J Cell Cloning 6:221
Lilly MB, Devlin PE, Devlin JJ, Rado TA (1987) Production of granulocyte colony-stimulating factor by a human melanoma cell line. Exp Hematol 15:966
Munker M, Munker R, Saxton RE, Koeffler HP (1987) Effect of recombinant monokines, lymphokines, and other agents on clonal proliferation of human lung cancer cell lines. Cancer Res 47:4081
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag, Berlin Heidelberg
About this paper
Cite this paper
Joraschkewitz, M. et al. (1990). Effects of Recombinant Cytokines on Clonogenic Cells from Primary Human Tumors. In: Freund, M., Link, H., Welte, K. (eds) Cytokines in Hemopoiesis, Oncology, and AIDS. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75510-1_26
Download citation
DOI: https://doi.org/10.1007/978-3-642-75510-1_26
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-52281-2
Online ISBN: 978-3-642-75510-1
eBook Packages: Springer Book Archive