Abstract
The ability to grow pure cultures of large vessel endothelial cells has resulted in a marked increase in our understanding of the function of these cells. For a long time endothelial cells were thought to be rather inert cells forming a physical barrier on the inside of blood vessels. There was very little appreciation of the complex metabolic activity that these cells carry out. It is now clear that in addition to actively participating in the regulation of the two main functions of the endothelium, that of a macromolecular barrier and of a thromboresistant surface [16], large vessel endothelial cells have a wide range of metabolic activities. Culture studies have also shown that the endothelial cells secrete components of the wall and substances that regulate smooth muscle cell function.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Booyse FM, Sedlak BJ, Rafelson ME (1975) Culture of arterial endothelial cells: characterization and growth of bovine aortic endothelial cells. Thromb Diath Haemorrh 34:825–839.
Dickinson ES, Slakey LL (1982) Plasma-derived serum as a selective agent to obtain endothelial cultures from swine aorta. In Vitro 18:63–70.
French JE, Jennings MA, Poole JCF, Robinson DS, Florey H (1963) Intimai changes in the arteries of ageing swine. Proc R Soc Lond [Biol] 158:24–42.
French JE, Jennings MA, Florey HW (1965) Morphological studies on atherosclerosis in swine. Ann NY Acad Sci 127:780–799.
Gerrity RG (1981) The role of the monocyte in atherogenesis. II. Migration of foam cells from atherosclerotic lesions. Am J Pathol 103:191–200.
Gerrity RG, Naito HK, Richardson M, Schwartz CJ (1979) Dietary induced atherogenesis in swine. Morphology of the intima in prelesion stages. Am J Pathol 95:775–792.
Giddings JC, Jarvis AL, Bloom AL (1983) Differential localization and synthesis of porcine factor VIII related antigen (VIII R:AG) in vascular endothelium and in endothelial cells in culture. Thomb Res 29:299–312.
Gimbrone MA, Cotran RS, Folkman J (1974) Human vascular endothelial cells in culture: growth and DNA synthesis. J Cell Biol 60:673–684.
Gotlieb AI, Spector W (1981) Migration into an experimental wound: a comparison of porcine aortic endothelial and smooth muscle cells and the effect of culture irradiation. Am JPathol 103:271–282.
Gotlieb AI, Subrahmanyan L, Kalnins VI (1983) Microtubule organizing centers and cell migration. Effect of inhibition of migration and microtubule disruption in endothelial culture. J Cell Biol 96:1266–1272.
Gotlieb AI, Spector W, Wong MKK, Lacey C (1984) In vitro reendothelialization: microfilament bundle reorganization in migrating porcine endothelial cells. Arteriosclerosis 4:91–96.
Gottlieb H, Laiich JJ (1954) The occurrences of arteriosclerosis in the aorta of swine. Am J Pathol 30:851–853.
Jaffe EA, Nachman RL, Becker CG, Mimick CR (1973) Culture of human endothelial cells derived from umbilical veins. J Clin Invest 52:2745–2756.
Koo EWY, Gotlieb AI (1989) Endothelial stimulation of intimai cell proliferation in a porcine aortic organ culture. Am J Pathol 134:497–503.
Magargal WW, Dickinson ES, Slakey LL (1978) Distribution of membrane marker enzymes in cultured arterial endothelial cells and smooth muscle cells. J Biol Chem 253:8311–8318.
Ryan US (ed) (1988) Endothelial cells, vols 1–3. CRC, Boca Raton.
Ryan US, Clements E, Habliston D, Ryan JW (1978) Isolation and culture of pulmonary artery endothelial cells. Tissue Cell 10:535–554.
Scott RF, Thomas WA, Lee WM, Reiner JM, Florentin RA (1979) Distribution of intimai smooth muscle cell masses and their relationship to early atherosclerosis in the abdominal aortas of young swine. Atherosclerosis 34:291–301.
Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G (1986) A monoclonal antibody against α-smooth muscle actin. A new probe for smooth muscle differentiation. J Cell Biol 103:2787–2796.
Slater DN, Sloan JM (1975) The porcine endothelial cell in culture. Atherosclerosis 21:259–272.
Thomas WA, Reiner JM, Florentin RA, Lee KT, Lee WM (1976) Population dynamics of arterial smooth muscle cells. V. Cell proliferation and cell death during initial 3 months in atherosclerotic lesions induced in swine by hypercholesterolemic diet and intimai trauma. Exp Mol Pathol 24:360–374.
Tsukada T, Rosenfeld M, Gown A (1986) Immunocytochemical analysis of cellular component in atherosclerotic lesions: use of monoclonal antibodies with the Watanabe and fat-fed rabbit. Arteriosclerosis 6:601–613.
Tsukada T, Tippens D, Gordon D, Ross R, Gown A (1987) A muscle-actin-specific monoclonal antibody: I Immunocytochemical and biochemical characterization. Am J Pathol 127:51–60.
Voyta JC, Via DP, Butterfield CE, Zetter BR (1984) Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J Cell Biol 99:2034–2040.
Wong MKK, Gotlieb AI (1986) Endothelial cell monolayer integrity. I. The characterization of the dense peripheral band of microfilaments. Arteriosclerosis 6:212–219.
Wu QY, Drouet L, Carrier JL, Rothschild C, Berard M, Rouault C, Caen JP, Meyer D (1987) Differential distribution of von Willebrand factor in endothelial cells: comparison between normal pigs and pigs with von Willebrand disease. Arteriosclerosis 7:47–54.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Rosenthal, A.M., Gotlieb, A.I. (1990). Macrovascular Endothelial Cells from Porcine Aorta. In: Piper, H.M. (eds) Cell Culture Techniques in Heart and Vessel Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75262-9_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-75262-9_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-75264-3
Online ISBN: 978-3-642-75262-9
eBook Packages: Springer Book Archive