Cell Culture Techniques in Heart and Vessel Research

1. Front Matter

   Pages I-XIII

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2. Heart Muscle Cells

   1. Front Matter

      Pages 1-1

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   2. Embryonic Chick Heart Muscle Cells

      • A. C. Nag

      Pages 3-19

   3. Neonatal Rat Heart Muscle Cells

      • A. Pinson

      Pages 20-35

   4. Adult Ventricular Rat Heart Muscle Cells

      • H. M. Piper, A. Volz, P. Schwartz

      Pages 36-60

   5. Adult Atrial Cardiocytes in Culture

      • M. Cantin

      Pages 61-74

   6. Muscle Cell Cultures from Human Heart

      • S. L. Jacobson, R. A. Altschuld, C. M. Hohl

      Pages 75-98

   7. Attachment Substrates for Heart Muscle Cells

      • T. K. Borg, L. Terracio

      Pages 99-107

   8. Microcarrier Culture of Neonatal Heart Cells

      • P. A. Uusimaa, I. E. Hassinen

      Pages 108-114

3. Endothelial Cells

   1. Front Matter

      Pages 115-115

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   2. Macrovascular Endothelial Cells from Porcine Aorta

      • A. M. Rosenthal, A. I. Gotlieb

      Pages 117-129

   3. Microvascular Endothelial Cells from the Lungs

      • U. S. Ryan

      Pages 130-139

   4. Microvascular Endothelial Cells from Brain

      • P. D. Bowman, M. du Bois, K. Dorovini-Zis, R. R. Shivers

      Pages 140-157

   5. Microvascular Endothelial Cells from Heart

      • H. M. Piper, R. Spahr, S. Mertens, A. Krützfeldt, H. Watanabe

      Pages 158-177

   6. Macro- and Microvascular Endothelial Cells from Human Tissues

      • V. W. M. van Hinsbergh, M. A. Scheffer, E. G. Langeler

      Pages 178-204

   7. Vascular Endothelial Cell-Synthesized Extracellular Matrices as Attachment Substrates In Vitro

      • I. M. Herman

      Pages 205-211

   8. Endothelial Cells Grown on Filter Membranes

      • D. M. Shasby, S. S. Shasby

      Pages 212-219

   9. Microcarrier Cultures of Endothelial Cells

      • R. Spahr, H. M. Piper

      Pages 220-229

   10. Growth Factors for Vascular Endothelial Cells

       • D. Gospodarowicz

       Pages 230-244

4. Smooth Muscle Cells and Pericytes

   1. Front Matter

      Pages 245-245

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In the cardiovascular sciences, an increasing demand for the use of modern methods of cell biology has developed. The use of specific cell culture models of the various tissues involved is essential for most of these novel approaches. This book meets the demand for acomprehensive and easy accessible source for cell cul- ture methods in cardiovascular research as it was not previously available. The basic methods for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), smooth muscle cells and pericytes are described in detail by an international selection of experts. Special chapters discuss the use of growth factors and attachment substrates, techniques for co-cultures, cultures on permeable filter membranes and microcarrier cultures. The methodological descriptions are sufficiently detailed for an immediate application in the laboratory. All chapters also contain a critical evaluation of alternative approaches.

• Endothelzellen
• Kardiomyozyten
• Myokardiozyten
• Myokardzell-Kultur
• cardiovascular
• cells
• endothelium
• glatte Muskelzellen
• smooth muscle
• tissue

• Physiologisches Institut, Abteilung für Herz- und Kreislaufphysiologie der Universität Düsseldorf, Düsseldorf 1, Germany

  H. M. Piper

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