Abstract
Cardiac myocytes in culture have been available for three decades [3, 6], but this methodological approach has been in its developmental stage until recent years. The culturing of striated cardiac myocytes even from the neonatal heart is hampered by a low biomass yield because of the limits of cell division [8] in cells derived from a tissue which is already amitotic 17 to 21 days postnatally [14]. Cultured heart cells have many advantages over newly isolated cells, however, because the latter are probably in an abnormal metabolic state after isolation due to reversible damage to the plasma membrane during tissue disaggregation. A marked difference is observed in Ca2+ tolerance, for example.
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© 1990 Springer-Verlag Berlin Heidelberg
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Uusimaa, P.A., Hassinen, I.E. (1990). Microcarrier Culture of Neonatal Heart Cells. In: Piper, H.M. (eds) Cell Culture Techniques in Heart and Vessel Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75262-9_7
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DOI: https://doi.org/10.1007/978-3-642-75262-9_7
Publisher Name: Springer, Berlin, Heidelberg
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