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Muscle Cell Cultures from Human Heart

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Cell Culture Techniques in Heart and Vessel Research

Abstract

There are few reports of work with isolated or cultured human cardiac muscle cells. With the exception of Bloom [3], early work was with cultures derived from prenatal material obtained at a variety of gestational times. At first cultures were prepared from explanted tissue; 4- to 14-week embryos [36], 8-to 12-week embryos [29], 6- to 8-week embryos [19], a 13-week embryo [11], and 7- to 10-week embryos [5]. Development of methods for isolation of single cells by treating tissue with enzymes made it possible to prepare cultures of isolated embryonic cells. Halbert et al. [14] and Thompson [35] prepared cultures of cells isolated from 13- to 20-week embryos and, similarly, Lieberman et al. [27] prepared cultures of isolated cells from 14- to 16-week embryos.

The section “Isolation of Human Ventricular Myocytes by Vascular Perfusion with Collagenase” was written by R. A. Altschuld and C. M. Hohl, Department of Physiological Chemistry, The Ohio State University, Columbus, OH 43210-1239, USA. SLJ’s work was supported by a grant from the Heart and Stroke Foundation of Ontario. Work described in “Isolation of Human Ventricular Myocytes by Collagenase Vascular Perfusion” was supported in part by grants in aid from the American Heart Association, Ohio Affiliate, and USPHS-HL36240

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Authors
  1. S. L. Jacobson
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  2. R. A. Altschuld
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  3. C. M. Hohl
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Editor information

Editors and Affiliations

  1. Physiologisches Institut, Abteilung für Herz- und Kreislaufphysiologie der Universität Düsseldorf, Moorenstr. 5, D-4000, Düsseldorf 1, Germany

    H. M. Piper

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© 1990 Springer-Verlag Berlin Heidelberg

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Jacobson, S.L., Altschuld, R.A., Hohl, C.M. (1990). Muscle Cell Cultures from Human Heart. In: Piper, H.M. (eds) Cell Culture Techniques in Heart and Vessel Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75262-9_5

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  • DOI: https://doi.org/10.1007/978-3-642-75262-9_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-75264-3

  • Online ISBN: 978-3-642-75262-9

  • eBook Packages: Springer Book Archive

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