Abstract
Vascular smooth muscle cells, like other anchorage-dependent cells, will die if maintained in suspension without attachment to a substratum. They are therefore usually cultured on tissue culture plastic (in flasks and Petri dishes) from which they can be released by the action of trypsin: EDTA. In contrast, the use of microcarrier beads provides a mobile substratum for cell growth and manipulation. Furthermore, microcarrier techniques provide a large surface area for endothelial culture in a small volume of beads, thereby allowing perfusion columns of microcarriers containing large numbers of smooth muscle cells to be constructed and, if scale-up of tissue culture is desired, batches of cells may be grown in suspension culture in the research laboratory on an economical scale.
This study was funded by grants to Dr. Davies from the National Heart, Lung, and Blood Institutes of the National Institutes of Health (HL 36049 and HL 36028)
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Davies, P.F., O’Connor, S.E. (1990). Methods of Culturing Vascular Smooth Muscle Cells on Microcarriers. In: Piper, H.M. (eds) Cell Culture Techniques in Heart and Vessel Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75262-9_22
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DOI: https://doi.org/10.1007/978-3-642-75262-9_22
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