Functional Dissociation of T Cell Sites; Immunogenic, Antigenic and Pathogenic Sites

  • Dale S. Gregerson
  • Steven P. Fling
  • Wesley F. Obritsch
  • Carmen F. Merryman
  • Larry A. Donoso
Conference paper
Part of the NATO ASI Series book series (volume 38)

Abstract

Experimental autoimmune uveoretinitis (EAU) is a CD4+ T-cell mediated autoimmune disease induced by S-antigen, a retinal photoreceptor cell protein. In order to identify T cell recognition sites responsible for pathogenicity, cyanogen bromide fragments and synthetic peptides were used to test uveitogenic T cell lines prepared against native S-antigen. Two non-overlapping synthetic peptides known to actively induce EAU did not induce proliferative responses in these T cell lines. In contrast, an adjacent synthetic peptide which was unable to actively induce EAU elicited strong proliferative responses from the T cell lines and gave rise to a uveitogenic line. Our results indicate that spatially distinct T cell epitopes are present in S-antigen which are responsible for the active induction of EAU, lymphocyte proliferation, and the ability to adoptively transfer EAU.

Keywords

Bromide Tuberculosis Uveitis Blindness Encephalomyelitis 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Dale S. Gregerson
    • 1
    • 2
  • Steven P. Fling
    • 2
  • Wesley F. Obritsch
    • 1
  • Carmen F. Merryman
    • 3
  • Larry A. Donoso
    • 4
  1. 1.Departments of OphthalmologyUniversity of MinnesotaMinneapolisUSA
  2. 2.Departments of MicrobiologyUniversity of MinnesotaMinneapolisUSA
  3. 3.Department of Biochemistry and Molecular BiologyThomas Jefferson UniversityPhiladelphiaUSA
  4. 4.Wills Eye Hospital Research DivisionPhiladelphiaUSA

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